Abstract
The maximum yield of β-glucosidase by A. niger KK2 mutant, grown on the basal medium for 7 days, was 514 I U g−1 ground rice straw, and was about twice those obtained from wheat straw or bran by previous researchers. Optimal activity of β-glucosidase was at 60–70 °C and pH 4.8.
Similar content being viewed by others
References
Anand L, Vithayathil PJ (1996) Xylan-degrading enzymes from the thermophilic fungus Humicola lanuginosa Bunce: Action pattern of xylanase and β-glucosidase on xylans, xylooligomers and arabinoxylooligomers. J. Ferment. Bioeng. 81: 511-517.
Bhat MK, Bhat S (1997) Cellulose degrading enzymes and their potential industrial application. Biotechnol. Adv. 15: 583-620.
Chnstakopoulos P, Kekos D, Kolisis FN, Macris BJ (1995) Controlling simultaneous production of endocellulase and β-glucosidase by Fusarium oxysporum in submerged culture. Biotechnol. Lett. 17: 883-888.
Doppelbauer R, Esterbauer H, Steiner W, Lafferty RM, Steinmuller H (1987) The use of lignocellulosic wastes for production of cellulase by Trichoderma reesei. Appl. Microbiol. Biotechnol. 26: 485-194.
Gokhale DV, Puntambekar US, Vyas AK, Patil SG, Deobagkar N (1984) Hyperproduction of β-glucosidase by an Aspergillus sp. Biotechnol. Lett. 6: 719-722.
Hoffman RM, Wood TM (1985) Isolation and partial characterization of a mutant of penicillium funiculosum for the saccharification of straw. Biotechnol. Bioeng. 27: 81-85.
Kang SW, Kim SW, Kim K (1994) Production of cellulase and xylanase by Aspergillus niger KKS. J. Microbiol. Biotechnol. 4: 49-55.
Kim SW, Moon KH, Tae BS (1996) Simple and rapid method for isolation of A. niger mutant with enhanced cellulase and xylanase. Biotechnol. Tech. 10: 735-736.
Macris BJ, Kekos D (1989) In: Coughlan MP, eds. Enzyme Systems for Lignocellulose Degradation, Elsevier Applied Science, pp. 262-271.
Persson I, Tjernld F, Hagerdal B (1991) Fungal cellulolytic enzyme production: A review. Process Biochem. 26: 65-74.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kang, S.W., Ko, E.H., Lee, J.S. et al. Over-production of β-glucosidase by Aspergillus niger mutant from lignocellulosic biomass. Biotechnology Letters 21, 647–650 (1999). https://doi.org/10.1023/A:1005556523241
Issue Date:
DOI: https://doi.org/10.1023/A:1005556523241