Abstract
Like many diapausing crustaceans, anostracans (Crustacea, Branchiopoda) produce encysted embryos capable of surviving the long periods of desiccation typical of their environments. These cysts are far more abundant and are easier to collect than adults and for some applications they are superior to adults as subjects for genetic analysis. Due to the lack of minimal tissue size requirements, PCR-based analysis is the best alternative for genetic study of cysts. We describe a method for the fast extraction of DNA from cysts, yielding template for RAPD-PCR analysis.
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References
Belk, D., 1977. Evolution of egg size strategies in fairy shrimps. S. West Nat. 22: 99–105.
Clegg, J. S. & F. P. Conte, 1980. A review of the cellular and developmental biology of Artemia. In Persoone, G., P. Sorgloos, O. Roels & E. Jaspers (eds). The Brine Shrimp Artemia. Vol. 2. Physiology, Biochemistry, Molecular Biology. Universa Press, Wetteren, Belgium: 11–54.
Davis, L. G., M. D. Dibner & J. F. Battey, 1986. Basic Methods in Molecular Biology. Elsevier Science Publishing Co., Inc., New York, 388 pp.
Lécher, P., D. Defaye & P. Noel, 1995. Chromosomes and nuclear DNA of Crustacea. Invert. Reprod. Dev. 27: 85–114.
Linder, H. J., 1960. Studies on the fresh water fairy shrimp Chirocephalopsis bundyi(Forbes). II. Histochemically of EggShell Formation. J. Morph. 107: 259–284.
Moorad, J.A, M.A. Simovich & M. S. Mayer, 1997. Identification of Southern Californian Branchinectid Cysts (Crustacea, Anostraca) using RAPD-PCR Species-Specific Markers. J. Crust. Biol. (In Press).
Saiki, R., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis & H. A. Erlich, 1988. Primerdirected enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.
Simovich, M. A. & S. A. Hathaway, 1997. Diversified bet-hedging as a reproductive strategy of some ephemeral pool anostracans (Branchiopoda). J. Crust. Biol. 17: 38–44.
Walsh, P. S., D. A. Metzger & R. Higuchi, 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 506–513.
Welsh, J. & M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213–7218.
Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski & S. V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.
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Moorad, J.A., Mayer, M.S. & Simovich, M.A. Extraction of DNA from anostracan cysts (Crustacea, Branchiopoda) for use in RAPD-PCR analyses. Hydrobiologia 359, 159–162 (1997). https://doi.org/10.1023/A:1003154618381
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DOI: https://doi.org/10.1023/A:1003154618381