Abstract
The allozyme variation of 15 Tunisian wild populations of Mentha pulegium L. threatened by human activities (clearing, hard-grazing, ploughing, traditional uses) was surveyed by the analysis of 14 isozyme loci using horizontal gel starch electrophoresis. The species exhibited a high level of genetic variation within populations (the mean Ap = 2.20, P = 72%, Ho = 0.349 and He = 0.229), which indicates a predominately outcrossing mating system and the recruitment of new genotypes via dispersal seeds. The genetic structure analysis of the populations using F statistics indicates no inbreeding, and showed an excess of heterozygosity for few loci. The moderate differentiation of populations (FST = 0.110) and the low rate of gene flow between them (Nm = 2.02) might been caused by recent isolation of the populations through biotope disturbances. The value of Nei's genetic identity varied from 0.839 to 0.999 reflecting a relatively low genetic divergence between populations. Cluster analysis using UPGMA method and Nei's genetic identity values, showed that populations geographically close didn't always cluster together. However, populations within the same bioclimatic stage generally subclustered together indicating that differentiation between bioclimatic regions occurred.
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Baatout H., Marrakchi M. and Combes D. 1991. Genetic diver-gence and allozyme variation within and among populations of Hedysarum spinosissimum subsp. capitatum and subsp. spinosissimum (Papillonaceae). Taxon 40: 239-252.
Bartlett E., Novak S.J. and Mack R.N. 2002. Genetic variation in Bromus tectorum (Poaceae). differentiation in the Eastern United States. American Journal of Botany 89: 602-612.
Ben Fadhel N. and Boussaid M. 2002. Genetic diversity in Mentha 'eme' pulegium L. 13 journees de la A.T.S.B. Hammamet (Tunisie) Mars 2002.
Bingham R.A. and Ranker T.A. 2000. Genetic diversity in alpine and foot hill populations of Campanula rotundifolia (Campanulaceae). International Journal of Plant Sciences 161: 403-411.
Boussa?d M., Ben Fadhel N., Trifi-Farah N., Abdelkefi A. and Marrakchi M. 1995. Les especes mediterraneennes du genre Hedysarum L. In: Prosperi J.M., Guy P. and Balfourier F. (eds), Ressources genetiques des plantes fourageres et a gazon. Bureau des Ressources Genetiques, Paris, France, pp. 115-130.
Boussa?d M., Ben Fadhel N., Chemli R. and Ben M'hamed M. 1998. Structure of vegetation in Northern and Central Tunisia and protective measures. Cahiers Options Mediterraneennes 38: 295-302.
Brun N., Colson M., Perrin A. and Voirin B. 1991. Chemical and morphological studies of the effects of aging on monoterpene composition in Mentha x piperita leaves. Canadian Journal of Botany 69: 2271-2278.
Bruneton J. 1993. Pharmacognosie, phytochimie, plantes medicinales. 2'eme' edition. Technique et Documentation (eds), Paris, France, p. 558.
Caissard J.C., Faure O., Julien F., Colson M. and Perrin A. 1996. Direct regeneration in vitro and transient GUS expression in Mentha x Piperita. Plant Cell Reports 16: 67-70.
Chambers H. 1992. Mentha. genetic resources and the collection at USDA-ARSNCGR-Corvallis. Lamiales News letter 1: 3-4.
Chung M.G. 1994. Genetic structure in Korean populations of Hosta capitata (Liliaceae). Journal of Plant Biology 37: 277-284.
Crawford D.J. 1989. Enzyme electrophoresis and plant systematics. In: Soltis D.E. and Soltis P.S. (eds), Isozymes in plant biology. Discorides, Portland, Oregon, pp. 146-164.
Ellstrand N.C. and Roose M.L. 1987. Patterns of genotypic diversity in clonal plant species. American Journal of Botany 74: 123-131.
Emberger L. 1966. Une classification biogeographique des climats. In: Recherches et Travaux des Laboratoires de Geologie, Botanique et Zoologie. Faculte des Sciences Mon-tpellier, France Vol. 7, pp. 1-43.
Evans M.E.K., Dolan R.W., Menges E.S. and Gordon D.R. 2000. Genetic diversity and reproductive biology in Warea carteri (Brassicaceae), a narrowly endemic Florida scrub annual. American Journal of Botany 87: 372-381.
Faure O., Diemer F., Moja S. and Jullien F. 1998. Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks. Plant Cell, Tissue and Organ Culture 52: 209-212.
Francisco-Ortega J., Santos-Guerra A., Kim S.C. and Crawford D.J. 2000. Plant genetic diversity in the Canary Island. a conservation perspective. American Journal of Botany 87: 909-919.
Gao L.Z., Ge S. and Hong D.Y. 2000. Low levels of genetic diversity within populations and high differentiation among populations of wild Rice, Oryza granulata. Nees et Arn. ex Watt., from China. International Journal of Plant Sciences 161: 691-697.
Godt M.J. and Hamrick J.L. 1993. Genetic diversity and popula-tion stucture in Tradescantia hirsuticaulus (Commenlinaceae). American Journal of Botany 80: 959-966.
Godt M.J.W., Johnson B.R. and Hamrick J.L. 1996. Genetic diversity and population size in four rare southern Appalachian plant species. Conservation Biology 10: 796-805.
Gottlieb L.D. 1977. Electrophoretic evidence and plant systematics. Annals of the Missouri Botanical Garden 64: 161-180.
Hammer K. 2001. Mentha. In: Hanelt P. and Institute of Plant Genetics and Crop Plant Research (eds), Mansfeld's Encyclo-pedia of Agricultural and Horticultural Crops. Springer Verlag, Berlin, Germany.
Hamrick J.L., Linhart Y.B. and Mitton J.B. 1979. Relationship between life history characteristic and electrophoretically de-tectable genetic variation in plants. Annual Review of Ecology and systematics 10: 173-200.
Hamrick J.L. and Godt M.J.W. 1989. Allozyme diversity in plant species. In: Brown A.H.D., Clegg M.T., Kahler A.L. and Weir B.S. (eds), Plant Population Genetics, Breeding and Germplasm Resources. Sinauer, Sunderland, Massachusetts, USA, pp. 43-63.
Hamrick J.D. and Godt M.J.W. 1996. Conservation genetics of endemic plant species. In: Avise J.C. and Hamrick J.L. (eds), Conservation genetics. case histories from nature. Chapman and Hall, New York, USA, pp. 281-304.
Hannan G.L. and Orick M.W. 2000. Isozyme diversity in Iris Cristata and the threatened glacial endemic I. lacustris (Iridaceae). American Journal of Botany 87: 153-168.
Harley R.M. and Brighton C.A. 1977. Chromosome numbers in the genus Mentha L. Botanic Journal of Linn. Soc. 74: 71-76.
Kang S.S., Noguchi J., Park K.B. and Chung M.Gi. 1998. Allozyme diversity in Japanese population of Hemerocallis thunbergii, H. middendorffii, and H. exaltata (Liliaceae). Nordic Journal of Botany 18: 581-587.
Lange B.M. and Croteau R. 1999. Genetic engineering of essential of production in Mint. Current opinion in Plant Biology 2: 139-144.
Le Floc'k E. 1983. Contribution a une etude ethnobotanique de la flore tunisienne. 216-217. I.O.R (eds), Tunisie, p. 542.
Lewis P.O. and Crawford D.J. 1995. Pleistocene refugium en-demic exhibit greater allozymic diversity than widespread congeners in the genus Polygonella (Polygonaceae). American Journal of Botany 82: 141-149.
Matolweni L.O., Balkwill K. and Mc Lellan T. 2000. Genetic diversity and gene flow in the morphologically variable, rare endemics Begonia dregei and Begonia homonyma (Begoniaceae). American Journal of Botany 87: 431-439.
Medou G., Faure O., Jullien F., Colson M. and Perrin A. 1997. Caulogenese et synthese terpenique in vitro chez la Menthe poivree. Acta Bot. Gallica 144: 371-379.
Neel M.C. and Ellstrand N.C. 2001. Patterns of allozyme diversity in the threatened plant Erigeron parishii (Asteraceae). American Journal of Botany 88: 810-818.
Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
Panneta F.D. 1986. Chromosome numbers in Western Australian populations of pennyroyal (Mentha pulgium). Plant Protection Quarterly 1: 106-110.
Pappert R.A., Hamrick J.L. and Donovan L.A. 2000. Genetic variation in Pueraria lobata (Fabaceae), an introduced, clonal, invasive plant of the Southeastern United States. American Journal of Botany 87: 1240-1245.
Pasteur N., Pasteur G., Bonhomme F., Catalan J. and Britton-Davidian J. 1987. Manuel technique de genetique par electrophorese des proteines. Technique et Documentation (Lavoisier) (eds), Paris, France.
Pernes J. 1984. Gestion des ressources genetiques des plantes. Tome II. Technique et Documentation (Lavoisier) (eds), Paris, France.
Peyron L. 1985. Aspects technico-economiques et commerciaux mondiaux des huiles essentielles de Menthe. Actes du colloque ''Les menthes en France. Aspects scientifiques, economiques et industriels'' Lyon, France, pp. 87-120.
Pottier-Alapetite G. 1979. Flore de la Tunisie, Angiospermes dicotyledones. Apetales-Dialypetales. I.O.R. (eds), Tunisie, p. 654.
Quezel P. and Santa S. 1962. Nouvelle flore de l'Algerie et des regions desertiques meridionales, T:1. C.N.R.S. (eds), Paris, France.
Richards A.J. 1986. Plant Breeding Systems. George Allen and Unwin, London, UK.
Salhi-Hannachi A., Boussaid M. and Marrakchi M. 1998. Genetic variability organisation and gene flow in natural populations of Medicago polymorpha L. prospected in Tunisia. Genetics Selection Evolution 30: 121-135.
Simon J.E., Chadwick and Craker L.E. 1984. Herbs: An Indexed Bibliography 1971-1981. The scientific literature on selected herbs, and aromatic and medicinal plants of temperate zone. Archon Books, Hamden, CT.
Singh T.P. 1984. Cytological studies in Mentha and Salvia as correlated with their chemical contents. Indian J. of Heredity 16: 30-60.
Soltis D.E., Haufler C.H., Darrow D.C. and Gastony G.J. 1983. Starch gel electrophoresis of ferns. a compilation of grinding buffers, gel and electrode buffers, and staining schedules. American Fern Journal 63: 9-27.
Soltis P.S., Soltis D.E. and Holsinger K.E. 1988. Estimates of intragametophytic selfing and interpopulational gene flow in Homosporous ferns. American Journal of Botany 75: 1765-1765.
Soltis P.S. and Soltis D.E. 1991. Genetic variation in endemic and widespread plant species, examples from Saxifragaceae and Polystiochum (Dryopteridaceae). Aliso 13: 215-223.
Stebbins G.L. 1974. Flowering plants: Evolution Above the Species Level. Belknap Press of Harvard University Press, Cambridge, Massachusetts, USA.
Stuber C.W., Goodman M.M. and Johson F.M. 1977. Genetic control and racial variation of ß-glucosidase isozymes in maize (Zea mays). Biochemical Genetics 15: 383-394.
Swofford D.L. and Selander R.B. 1981. Biosys-1: A computer program for the analysis of allelic variation in population genetics and biochemical systematics. Illinois Natural History Survey, Champaign, IL.
Tarayre M. and Thompson J.D. 1996. Population genetic structure of the gynodioecious Thymus vulgaris (labiateae) in Southern France. Journal of evolutionary biology 10: 157-174.
Van Eck J.M. and Kitto S.L. 1990. Callus initiation and regenera-tion in Mentha. Hort Science 25: 804–806.
Voirin B., Bayet C., Faure O. and Jullien F. 1999. Free Flavonoid aglycones as markers of percentage in Mentha aquatica, M. citrata M. spicata and M. x piperita. Phytochemistry 50: 1189–1193.
Wendel J.F. and Weeden N.F. 1989. Visualization and interpreta-tion of plant isozymes. In: Soltis D.E. and Soltis P.S. (eds), Isozymes in plant biology. Discorides Press, Portland, Oregan, USA, pp. 5–45.
Workman P.L. and Niswander J.D. 1970. Population studies on Southwestern Indian tribes. II. Local genetic differentiation in the Papago. American Journal of Human Genetics 22: 24–49.
Wright S. 1951. The genetic structure of populations. Annals of Eugenics 15: 323–354.
Wright S. 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19: 395–420.
Wright S. 1978. Evolution and the genetics of populations,Vol. 4, Variability within and among natural populations. University of Chicago Press, Chicago, Illinois, USA.
Ziadi F., Voirin B., Jay M. and Virial M.R. 1998. Free flavonoid aglycones from leaves of Mentha pulgium and Mentha suaveolens (Labiatae). Phytochemistry 48: 991–994.
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Fadhel, N.B., Boussaïd, M. Genetic diversity in wild Tunisian populations of Mentha pulegium L. (Lamiaceae). Genetic Resources and Crop Evolution 51, 309–321 (2004). https://doi.org/10.1023/B:GRES.0000024016.13743.8b
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DOI: https://doi.org/10.1023/B:GRES.0000024016.13743.8b