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IBIS – A tool for automated sequential assignment of protein spectra from triple resonance experiments

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Abstract

We have developed a tool for computer-assisted assignments of protein NMR spectra from triple resonance data. The program is designed to resemble established manual assignment procedures as closely as possible. IBIS exports its results in XEASY format. Thus, using IBIS the operator has continuous visual and accounting control over the progress of the assignment procedure. IBIS achieves complete assignments for those residues that exhibit sequential triple resonance connectivities within a few hours or days.

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References

  • Bartels, C., Xia, T., Billeter, M., Güntert, P. and Wüthrich, K. (1995) J. Biomol. NMR, 5, 1–10.

    Google Scholar 

  • Dötsch, V., Matsuo, H. and Wagner, G. (1996) J. Magn. Reson., B112, 95–100.

    Google Scholar 

  • Eccles, C., Guntert, P., Billeter, M. and Wüthrich, K. (1991) J. Biomol. NMR, 1, 111–130.

    Google Scholar 

  • Friedrichs, M.S., Mueller, L. and Wittekind, M. (1994) J. Biomol. NMR, 4, 703–726.

    Google Scholar 

  • Hare, B.J. and Prestegard, J.H. (1994) J. Biomol. NMR, 4, 35–46.

    Google Scholar 

  • Ikura, M., Kay, L.E. and Bax, A. (1990) Biochemistry, 29, 4659–4667.

    Google Scholar 

  • Johnson, B.A. and Blevins, R.A. (1994) J. Biomol. NMR, 4, 603–614.

    Google Scholar 

  • Kay, L.E., Ikura, M., Tschudin, R. and Bax, A. (1990) J. Magn. Reson., 89, 496–514.

    Google Scholar 

  • Kraulis, P.J. (1994) J. Mol. Biol., 243, 696–718.

    Google Scholar 

  • Leutner, M., Gschwind, R.M., Liermann, J., Schwarz, C., Gemmecker, G. and Kessler, H. (1998) J. Biomol. NMR, 11, 31–43.

    Google Scholar 

  • Lukin, J.A., Gove, A.P., Talukdar, S.N. and Ho, C. (1997) J. Biomol. NMR, 9, 151–166.

    Google Scholar 

  • Meadows, R.P., Olejniczak, E.T. and Fesik, S.W. (1994) J. Biomol. NMR, 4, 79–96.

    Google Scholar 

  • Montelione, G.T. and Wagner, G. (1989) J. Am. Chem. Soc., 111, 5474–5475.

    Google Scholar 

  • Montelione, G.T. and Wagner, G. (1990) J. Magn. Reson., 87, 183–188.

    Google Scholar 

  • Moseley, H.N., Monleon, D. and Montelione, G.T. (2001) Meth. Enzymol., 339, 91–108.

    Google Scholar 

  • Nelson, S.J., Schneider, D.M. and Wand, A.J. (1991) Biophys. J., 59, 1113–1122.

    Google Scholar 

  • Olson, Jr. J.B. and Markley, J.L. (1994) J. Biomol. NMR, 4, 385–410.

    Google Scholar 

  • Oschkinat, H. and Croft, D. (1994) Meth. Enzymol., 239, 308–318.

    Google Scholar 

  • Oschkinat, H., Holak, T.A. and Cieslar, C. (1991) Biopolymers, 31, 699–712.

    Google Scholar 

  • Ou, H.D., Lai, H.C., Serber, Z. and Dötsch, V. (2001) J. Biomol. NMR, 21, 269–273.

    Google Scholar 

  • Roehrl, M.H.A., Hyberts, S.G., Sun, Z.-Y.J., Fields, A.P. and Wagner, G. (2003) J. Biomol. NMR, 26, 373–374.

    Google Scholar 

  • Schubert, M., Oschkinat, H. and Schmieder, P. (2001a) J. Biomol. NMR, 20, 379–384.

    Google Scholar 

  • Schubert, M., Oschkinat, H. and Schmieder, P. (2001b) J. Magn. Reson., 148, 61–72.

    Google Scholar 

  • Seavey, B.R., Farr, E.A., Westler, W.M. and Markley, J.L. (1991) J. Biomol. NMR, 1, 217–236.

    Google Scholar 

  • Wagner, G. and Wüthrich, K. (1982) J. Mol. Biol., 155, 347–366.

    Google Scholar 

  • Wüthrich, K., Wider, G., Wagner, G. and Braun, W. (1982) J. Mol. Biol., 155, 311–319.

    Google Scholar 

  • Xu, J., Straus, S.K., Sanctuary, B.C. and Trimble, L. (1994) J. Magn. Reson., B103, 53–58.

    Google Scholar 

  • Zimmerman, D., Kulikowski, C., Wang, L., Lyons, B. and Montelione, G.T. (1994) J. Biomol. NMR, 4, 241–256.

    Google Scholar 

  • Zimmerman, D.E. and Montelione, G.T. (1995) Curr. Opin. Struct. Biol., 5, 664–673.

    Google Scholar 

  • Zimmerman, D.E., Kulikowski, C.A., Huang, Y., Feng, W., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R. and Montelione, G.T. (1997) J. Mol. Biol., 269, 592–610.

    Google Scholar 

  • Zimmerman, D.E., Kulikowski, C.A. and Montelione, G.T. (1993) Proc. Int. Conf. Intell. Syst. Mol. Biol., 1, 447–455.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts MA,  02115, U.S.A

    Sven G. Hyberts & Gerhard Wagner

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  1. Sven G. Hyberts
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  2. Gerhard Wagner
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Hyberts, S.G., Wagner, G. IBIS – A tool for automated sequential assignment of protein spectra from triple resonance experiments. J Biomol NMR 26, 335–344 (2003). https://doi.org/10.1023/A:1024078926886

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