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Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobactersp. ATCC 53627 and its expression in Escherichia coli

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Abstract

A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.

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Authors and Affiliations

  1. Department of Bacterial Gene Technology, Novo Nordisk A/S, Bagsvaerd, Denmark

    S.T. Jørgensen & P.L. Jørgensen

  2. Biological Sciences Department, Heriot-Watt University, Riccarton Edinburgh, EH14 4AS, Scotland, UK

    M. Tangney

  3. NN Entotech, Inc., Davis, CA, USA

    R.L. Starnes

  4. National Institutes of Health, Bethesda, MD, USA

    R.L. Starnes

Authors
  1. S.T. Jørgensen
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  2. M. Tangney
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  3. R.L. Starnes
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  4. K. Amemiya
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  5. P.L. Jørgensen
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Jørgensen, S., Tangney, M., Starnes, R. et al. Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobactersp. ATCC 53627 and its expression in Escherichia coli. Biotechnology Letters 19, 1027–1031 (1997). https://doi.org/10.1023/A:1018459703742

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  • DOI: https://doi.org/10.1023/A:1018459703742

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