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Large-scale separation of magnetic bioaffinity adsorbents

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Abstract

Flat magnetic separator was used to separate magnetic bioaffinity adsorbents from litre volumes of suspensions. Both magnetic cross-linked erythrocytes and magnetic chitosan were efficiently separated; at least 95% adsorbent recovery was achieved at maximum flow rate (1680 ml min−1). Using this system low amounts of trypsin were concentrated from large sample volumes using magnetic erythrocytes as affinity adsorbent.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Microbiology, Institute of Landscape Ecology, Na Sádkách 7, 370 05, České Budějovice, Czech Republic

    Ivo Šafařík, Lucie Ptáčková & Mirka Šafaříková

Authors
  1. Ivo Šafařík
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  2. Lucie Ptáčková
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  3. Mirka Šafaříková
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Šafařík, I., Ptáčková, L. & Šafaříková, M. Large-scale separation of magnetic bioaffinity adsorbents. Biotechnology Letters 23, 1953–1956 (2001). https://doi.org/10.1023/A:1013742002533

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  • DOI: https://doi.org/10.1023/A:1013742002533

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