Abstract
An advanced solid-state fermentation (SSF) system (liquid medium absorbed on an inert support) has been applied to antibiotic production. The main components of this solid medium are: support (sugarcane bagasse), nutrients and water. The first two are solids and have to be considered to calculate the initial moisture content (IMC) of the medium. Earlier work indicated the importance of using high IMCs and concentrated media to obtain high penicillin yields in SSF. Nevertheless, the present work shows that high values of IMC or nutrients content can stimulate or inhibit penicillin production, depending on the strategy used to compensate this change (i.e. the proportions of the other two components). Conversely, increasing bagasse content always showed an inhibitory effect on the production. Since penicillin production depends on the combinations of these components, a global approach was used. The effect of the proportions of the three components on penicillin production was studied by means of a triangle of combinations and a 3D graph. It was possible to establish that high penicillin production is only obtained in a zone of low support content (10–12.5%). Surprisingly, one production maximum was observed in a zone of low moisture and high nutrients content (62 and 25.5% respectively); and another one in a zone of high moisture and a relatively low nutrients concentration (75.5 and 12.4% respectively).
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References
Acuña-Arguelles, M., Gutiérrez-Rojas, M., Viniegra-González, G. & Favela-Torres, E. 1993 Effect of water activity on exo-pectinase production by Aspergillus niger CH4 on solid state fermentation. Biotechnology Letters 16, 23–28.
Barrios-González, J. & Mejía, A. 1996 Secondary metabolites production by solid state fermentation. Biotechnology Annual Review 2, 85–121.
Barrios-González, J., Castillo, T.E. & Mejía, A. 1993a Development of high penicillin producing strains for solid state fermentation. Biotechnology Advances 11, 539–547.
Barrios-González, J., González, H. & Mejía, A. 1993b Effect of particle size, packing density and agitation on penicillin production in solid state fermentation. Biotechnology Advances 11, 525–537.
Barrios-González, J., Tomasini, A., Viniegra-González, G. & López, L. 1988 Penicillin production by solid state fermentation. Biotechnology. Letters 10, 793–798.
Hesseltine, C.W. 1977 Solid state fermentation Part 2, Process Biochemistry 12, 29–32.
Lonsane, B.K., Ghildyal, N.P., Budiatman, S. & Ramakrishna, S.V. 1985 Engineering aspects of solid state fermentation. Enzyme and Microbia Technology 7, 258–265.
Mitchel, D.A., Lonsane, B.K., Durand, A., Renaud, R., Almanza, S., Maratray, J., Desgranges, C., Crooke, K., Hong, K., Tanner, R.D. & Malaney, G.W. 1992 General principles of reactor design and operation for SSC. In Solid Substrate Cultivation, eds. Doelle, H.W., Mitchell, D.A. & Rolz, C.E. pp. 115. London and New York: Elsevier. ISBN 1–85166879–9.
Mudgett, R.E. 1986 Solid-state fermentation. In Manual of Industrial Microbiology and Biotechnology, eds. Demain, A.L. & Solomon, N.A. pp. 66–83 Washington: American Society for Microbiology. ISBN 0–91482673–5.
Oriol, E., Schettino, B., Viniegra-González, G. & Raimbault, M. 1988 Solid state culture of Aspergillus niger on support, Journal of Fermentation Technology 66, 57–62.
Pirt, S.J. & Righelato, R.C. 1967 Effect of growth rate on the synthesis of penicillin by Penicillium chrysogenum in batch and chemostat cultures. Applied Microbiology 15, 1284–1290.
Prior, B.A., Du Preez, J.C. & Rein, P.W. 1992 Environmental parameters. In Solid Substrate Cultivation, eds. Doelle, H.W., Mitchell, D.A. & Rolz, C.E. pp. 65 London and New York: Elsevier. ISBN 1–85166879–9.
Raimbault, M., Roussos, S., Barrios-González, J., Gutiérerez, M. & Viniegra-González, G. 1989 Procedeé de culture de microorganisms sur un milleu solide constituée d' un support solide absorbant, compressible et non fermentable. PATENTE (Brevet d' Invention) No. 2 647 119 Paris, France.
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Domínguez, M., Mejía, A., Revah, S. et al. Optimization of bagasse, nutrients and initial moisture ratios on the yield of penicillin in solid-state fermentation. World Journal of Microbiology and Biotechnology 17, 751–756 (2001). https://doi.org/10.1023/A:1012923814261
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DOI: https://doi.org/10.1023/A:1012923814261