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Effective selection and regeneration of transgenic rice plants with mannose as selective agent

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Abstract

A new method for the selection of transgenic rice plants without the use of antibiotics or herbicides has been developed. The phosphomannose isomerase (PMI) gene from Escherichia coli has been cloned and consitutively expressed in japonica rice variety TP 309. The PMI gene was transferred to immature rice embryos by Agrobacterium-mediated transformation, which allowed the selection of transgenic plants with mannose as selective agent. The integration and expression of the transgene was confirmed by Southern and northern blot analysis and the activity of PMI indirectly proved with the chlorophenol red assay. The results of genetic analysis showed that the transgenes were segregated in a Mendelian fashion in the T1 generation. The establishment of this selection system in rice provides an efficient way for producing transgenic plants without using antibiotics or herbicides with a transformation frequency of up to 41%.

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Author notes

  1. Xudong Ye

    Present address: Agracetus Campus, Monsanto Company, University Green, 8520 , Middleton, WI , 53562, U.S.A

Authors and Affiliations

  1. Institute for Plant Science, Swiss Federal Institute of Technology (ETHZ), Universitätsstrasse 2, 8092 , Zürich, Switzerland

    Paola Lucca & Ingo Potrykus

  2. Agracetus Campus, Monsanto Company, University Green, 8520 , Middleton, WI , 53562, U.S.A

    Ingo Potrykus

Authors
  1. Paola Lucca
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  2. Xudong Ye
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  3. Ingo Potrykus
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Lucca, P., Ye, X. & Potrykus, I. Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Molecular Breeding 7, 43–49 (2001). https://doi.org/10.1023/A:1009661014167

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