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High-resolution mapping adjacent to the Pc71 crown-rust resistance locus in hexaploid oat

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Abstract

The D526-derived BC1F2 population of hexaploid oat segregates for resistance to crown rust isolate 345. A mapping population consisting of 440 F2 individuals was used to develop a high-resolution RFLP map of the area of the genome where Pc71, the locus conferring this resistance, is located. Twelve RFLP markers have been identified within ca. 6 cM of Pc71, with cosegregating loci Xcdo1502 and Xcdo783 positioned 0.2 cM from the locus. All of the RFLP markers map to the same side of the locus, suggesting either that the Pc71 resistance locus resides at the end of a linkage group, or that there is no detectable heterozygosity on the opposite side. Some degree of microcollinearity between rice and oat is present in this area, as the two markers, Xcdo783 and Xrz69, linked to Pc71 are linked also in rice; however the genetic distance in oat is much less than the genetic distance in rice (6.2 cM and 20 cM, respectively).

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Author notes

  1. Arla L. Bush

    Present address: Department of Plant Pathology, Iowa State University, Ames, IA, 50011, USA (author for correspondence; e-mail

  2. Roger P. Wise

    Present address: Interdepartmental Genetics Program, Iowa State University, Ames, IA, 50011, USA

Authors and Affiliations

  1. Corn Insects and Crop Genetics Research Unit, USDA-Agricultural Research Service, Iowa State University, Ames, IA, 50011, USA

    Arla L. Bush & Roger P. Wise

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  1. Arla L. Bush
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Bush, A.L., Wise, R.P. High-resolution mapping adjacent to the Pc71 crown-rust resistance locus in hexaploid oat. Molecular Breeding 4, 13–21 (1998). https://doi.org/10.1023/A:1009652222382

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  • DOI: https://doi.org/10.1023/A:1009652222382

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