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Expressed sequence tags (ESTs) from the marine red alga Gracilaria gracilis

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Abstract

Expressed sequence tags (ESTs) are partial sequences of cDNAs, and can be used to characterize gene expression in organisms or tissues. We have constructed a 200-sequence EST database from vegetative thalli of Gracilaria gracilis, the first ESTs reported from any alga. This database contains recognizable ESTs corresponding to genes of carbohydrate metabolism (seven), amino acid metabolism (three), photosynthesis (five), nucleic acid synthesis, repair and processing (three), protein synthesis (14), protein degradation (six), cellular maintenance and stress response (three), other identifiable protein-coding genes (13) and 146 sequences for which significant matches were not found in existing sequence databases. We have already used this EST database to recover genes of carbohydrate biosynthesis from G. gracilis.

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Author notes

  1. Arturo O. Lluisma

    Present address: Marine Science Institute, University of the Philippines, 1101, Quezon City, Philippines

Authors and Affiliations

  1. Institute for Marine Biosciences, National Research Council (Canada), 1411 Oxford St, Halifax, NS, Canada, B3H 3Z1

    Arturo O. Lluisma & Mark A. Ragan

  2. Department of Biology, Dalhousie University, Halifax, NS, Canada, B3H 4J1

    Arturo O. Lluisma

  3. Program in Evolutionary Biology, Canadian Institute for Advanced Research, Canada

    Mark A. Ragan

Authors
  1. Arturo O. Lluisma
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  2. Mark A. Ragan
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Lluisma, A.O., Ragan, M.A. Expressed sequence tags (ESTs) from the marine red alga Gracilaria gracilis. Journal of Applied Phycology 9, 287–293 (1997). https://doi.org/10.1023/A:1007920607900

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