Abstract
Francisella tularensis subsp. tularensis is the common causal agent of tularemia in the USA and Canada, while F. tularensis subsp. palaearctica (holarctica) occurs in Europe, Asia, and to a minor extent in North America. F. tularensis subsp. mediaasiatica was found only in central Asia in a part of the former Soviet Union. Of the total of 155 F. tularensis strains isolated over the years 1978–1996 during the surveillance of tularemia in Slovakia, 65 were from small mammals, 68 from ticks and 22 from mites and fleas. They were characterized and classified by basic markers of infraspecific taxonomy in tests in vitro and compared with type strains of three subspecies and biovars of F. tularensis. Comparative studies have revealed biological properties characteristic of F. tularensis subsp. tularensis in 17 strains isolated from fleas and mites parasiting on small terrestrial mammals, collected in the Danube region, near Bratislava. These strains fermented glycerol, glucose, were positive for citrulline ureidase and sensitive to erythromycin, in contrast to the other 138 isolates classified as F. tularensis subsp. palaearctica (holarctica), biovar II, which fermented only glucose, were negative for citrulline ureidase and resistant to erythromycin. Two selected pairs of isolates with properties characteristic of F. tularensis subsp. palaearctica (holarctica), biovar II (SE-210, SE-234) and of F. tularensis subsp. tularensis (SE-219, SE-221), as shown in tests in vitro, were further examined for their pathogenicity on white mice, guinea pigs and domestic rabbits. In tests of virulence on domestic rabbits, the isolates SE-210 and SE-234 had low pathogenicity, while the isolates SE-219 and SE-221 exhibited high pathogenicity, which along with their biochemical properties confirmed their identification as strains of F. tularensis subsp. tularensis. The first findings of the highly virulent strains of F. tularensis subsp. tularensis in Europe indicate a serious event from epidemiologic and epiozootologic aspects, requiring systematic surveillance.
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References
Olsufiev NG, Meshcheryakova IS. Infraspecific taxonomy of tularemia agent Francisella tularensis Mc Coy et Chapin. J Hyg Epidemiol Microbiol Immunol 1982; 26: 291-299.
Olsufiev NG, Meshcheryakova IS. Subspecific taxonomy of Francisella tularensis Mc Coy and Chapin 1912. Int J Syst Bacter 1983; 33: 872-874.
Eigelsbach HT, Mc Gann VG. Gram-negative aerobic rods and cocci. In: Krieg NR, Holt JG (eds), Bergey's manual of systematic bacteriology. Baltimore: Williams & Wilkins,1984: 394-399.
Olsufiev NG. Taxonomy and characteristic of the genus Francisella Dorofeev,1947. J Hyg Epidemiol Microbiol Immunol 1970; 14: 67-74.
Olsufiev NG, Meshcheryakova IS. Further study of the interspecific taxonomy of Francisella tularensis. Zh Mikrobiol Epidemiol Immunobiol 1981;10: 16-21.
Guryčová D. Biological properties of the strains F. tularensis isolated in Slovakia. Čs Epidemiol 1980; 29; 220-229.
Guryčová D, Lysý J, Lichard M, et al. Study of a natural focus of tularemia in the Small Carpathian mountains. Bratisl lek Listy 1982; 78:155-163.
Guryčová D, Výrosteková V. Study of the circulation of F. tularensis in natural foci of Zahorska lowland and possibilities to influence it. Entomol Probl 1989; 19: 319-345.
Výrosteková V, Řeháček J, Guryčová D, Kocianová E. Prevalence of Francisella tularensis in ticks of Slovakia. Modern Acarology 1991; 2: 55-59.
Guryčová D, Kocianová E, Výrosteková V, Řeháček J. Prevalence of ticks infected with Francisella tularensis in natural foci of tularemia in western Slovakia. Eur J Epidemiol 1995; 11: 469-474.
Lukáš B, Libich J. Modification of a simple blood agar plate for cultivation of Pasteurella tularensis. Čs Epidemoil 1962; 10: 290-297.
Mörner T, Mattsson R, Forsman M, et al. Mentification and classification of different isolates of Francisella tularensis. J Vet Med 1993; B40: 613-620.
Sandström G, Sjöstedt A, Forsman M, et al. Characterization and classification of strains of Francisella tularensis isolated in the Central Asian Focus of the Soviet Union and in Japan. J Clin Microbiol 1992; 30:172-175.
Olsufiev NG, Emelyanova OS, Dunayeva TN. Comparative study of strains of B. tularense in the old and new world and their taxonomy. J Hyg Epidemiol Microbiol Immunol 1959; 3:138-149.
Guryčová D. The study of serological properties of F. tularensis. Folia Fac Med Univ Comenianae Bratisl 1987; 25:121-186.
Petrov VG. On the role of ticks Hirstionyssus musculi Johnst. in the transmission and preservation of tularemia infection. Zool J 1963; 42:1031-1040.
Olsufiev NG, Dunayeva TN. Natural focality, epidemiology and prophylaxis of tularemia. Moscow: Medicina,1970: 269 pp.
Hopla CE. Experimental studies on tick transmission of tularemia organisms. Am J Hyg 1953; 58:101-118.
Hopla CE. The ecology of tularemia. Adv Vet Sci Comp Med 1974;18: 25-53.
Výrosteková V. Transstadial transmission of Francisella tularensis in ticks Ixodes ricinus infected in the larval stage. Čs Epidemiol 1993; 42: 71-75.
Výrosteková V. Transstadial transmission of Francisella tularensis in ticks Ixodes ricinus infected in the nymphal stage. Epidemiol Mikrobiol Imunol 1994, 43:166-170.
Forsman M, Sandström G, Jaurin B. Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis. Appl Environ Microbiol 1990; 56: 949-955.
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Guryčová, D. First isolation of Francisella tularensis subsp. tularensis in Europe. Eur J Epidemiol 14, 797–802 (1998). https://doi.org/10.1023/A:1007537405242
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DOI: https://doi.org/10.1023/A:1007537405242