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Potential tumour doubling time: determination of Tpot for various canine and feline tumours

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Abstract

Spontaneous tumours in dogs and cats are an excellent model for clinical human research, such as in developing proton conformation radiotherapy for humans. The kinetics of tumour cells can be used effectively to predict prognosis and response to therapy in patients with tumours. Knowledge of the kinetic parameters in these tumours is therefore important. In the present study the kinetic parameters evaluated included the labelling index (LI), relative movement (RM), mitotic index (MI), and potential doubling time (Tpot). These parameters were determined using in vivo labelling with bromodeoxyuridine, flow cytometry and histological preparation. Samples were obtained and evaluated from 72 dogs and 20 cats, presenting as patients in our clinic. Within the groups of epithelial and mesenchymal tumours from dogs and cats, the kinetic parameters LI, RM and MI were compared with Tpot. Significant correlations were observed for the comparison Tpot and LI. No correlation was found between Tpot and RM.

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REFERENCES

  • Alanen, K.A., Klemi, P.J., Joensuu, H., Kujari, H. and Pekkala, E., 1989. Comparison of fresh, ethanol-preserved, and paraffin-embedded samples in DNA flow cytometry. Cytometry, 10, 81-85

    Google Scholar 

  • Becton Dickinson, 1989. Monoclonal antibodies detecting cell proliferation and activation. Source Book Becton Dickinson Immunocytometry Systems, Mountain View, California, USA, Section 2.80.1-3.80.2

  • Begg, A.C., 1989. Derivation of cell kinetic parameters from human tumours after labelling with bromodeoxyuridine or iododeoxyuridine. British Journal of Radiology, 19, 113-119

    Google Scholar 

  • Begg, A.C., McNally, N.J., Shrieve, D.C. and Kärcher, H., 1985. A method to measure the duration of the DNA synthesis and the potential doubling time from a single sample. Cytometry, 6, 620-626

    Google Scholar 

  • Carter, O.W., Robinson, J.P. and Narayanan, P.K., 1993. Flow cytometry: theory and clinical applications. Proceedings of the American College of Veterinary Internal Medicine Forum, 11, 785-787

    Google Scholar 

  • Cooke, L.D., Cooke, T.G., Forster, G., Jones, A.S. and Stell, P.M., 1994. Prospective evaluation of cell kinetics in head and neck squamous carcinoma: the relationship to tumour factors and survival. British Journal of Cancer, 69, 717-720

    Google Scholar 

  • Denekamp, J., 1970. The cellular proliferation kinetics of animal tumours. Cancer Research, 30, 393-400

    Google Scholar 

  • Dewhirst, M.W., LaRue, S.M. and Gerweck, L., 1995. Tumor physiology and cell kinetics. Seminars in Veterinary Medicine and Surgery (Small Animal), 10, 148-157

    Google Scholar 

  • Dolbeare, F.A., Gratzner, H.G., Pallavicini, M.G. and Gray, J.W., 1983. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proceedings of the National Academy of Science of the USA, 80, 5573-5577

    Google Scholar 

  • Dressler, L.G., Seamer, L., Owens, M.A., Clark, G.M. and McGuire, W.L., 1987. Evaluation of a modeling system for S-phase estimation in breast cancer by flow cytometry. Cancer Research, 47, 5294-5302

    Google Scholar 

  • Fowler, J.F., 1986. Potential for increasing the differential response between tumours and normal tissues: can proliferation rate be used? International Journal of Radiation Oncology, Biology and Physiology, 12, 641-645

    Google Scholar 

  • Gillette, E.L., 1982. Spontaneous canine neoplasms as models for therapeutic agents. In: I.J. Fidler and R.J. White (eds), Design of Models for Testing Cancer Therapeutic Agents, (Van Nostrand Reinhold), 185-192

  • Giordano, M., Riccardi, A., Danova, M., Brugnatelli, S. and Mazzini, G., 1991. Cell proliferation of human leukemia and solid tumors studied with in vivo bromodeoxyuridine and flow cytometry. Cancer Detection and Prevention, 15, 391-396

    Google Scholar 

  • Gratzner, H.G., 1982. Monoclonal antibody to 5-bromo-and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science, 218, 474

    Google Scholar 

  • Holmgren, L., O'Reilly, M.S. and Folkman, J., 1995. Dormancy of micrometastases: balanced prolifera-tion and apoptosis in the presence of angiogenesis suppression. Nature Medicine, 1, 149-153

    Google Scholar 

  • Hughes, W.L., Commerford, S.L., Gitlin, O., Krüger, R.C., Schultze, B., Shah, V. and Reilly, P., 1964. DNA metabolism in vivo: I. Cell proliferation and death as measured by incorporation and elimination of IUdR. Federation Proceedings, 23, 640

    Google Scholar 

  • Kinsella, T.J., Russo, A., Mitchell, J.B., Rowland, J., Jenkins, J., Schwade, J., Meyers, C.E., Colins, J.M., Speyer, J., Kornblith, P., Smith, B., Kufta, C. and Gladstein, E.A., 1984. A phase I study of intermittent bromodeoxyuridine with conventional fractionated irradiation. International Journal of Radiation Biology and Physiology, 10, 69-76

    Google Scholar 

  • LaRue, S.M., Fox, M.H., Withrow, S.J., Powers, B.E., Straw, R.C., Côté, I.M. and Gillette, E.L., 1994. Impact of heterogeneity in the predictive value of kinetic parameters in canine osteosarcoma. Cancer Research, 54, 3916-3921

    Google Scholar 

  • Mitchell, J.B., Kinsella, T.J., Russo, A., McPherson, S., Rowland, J., Kornblith, P. and Gladstein, E., 1983. Radiosensitization of hematopoietic precursor cells (CFUs) in glioblastoma patients receiving inter-mittent intravenous infusions of bromodeoxyuridine (BUdR). International Journal of Radiation Oncology, Biology and Physiology, 9, 457-464

    Google Scholar 

  • Owen, L.N., 1980. TMN Classification of Tumours in Domestic Animals, ed. 1. (World Health Organization, Geneva)

    Google Scholar 

  • Popert, R.J.M., Joyce, D.J., Walmsley, B.H. and Coptcoat, M.J., 1993. Bromodeoxyuridine labelling of transitional cell carcinoma of the bladder - an index of recurrence? British Journal of Urology, 71, 279- 283

    Google Scholar 

  • Remvikos, Y., Vielh, P., Padoy, E., Benyahia, B., Voillemot, N. and Magdelënat, H., 1991. Breast cancer proliferation measured on cytological samples: a study by flow cytometry of S-phase fractions and BrdU incorporation. British Journal of Cancer, 64, 501-507

    Google Scholar 

  • Rew, D.A., Wilson, G.D., Taylor, I. and Weaver, P.C., 1991. Proliferation characteristics of human colorectal carcinomas measured in vivo. British Journal of Surgery, 78, 60-66

    Google Scholar 

  • Rew, D.A., Campbell, I.D., Taylor, I. and Wilson, G.D., 1992. Proliferation indices of invasive breast carcinomas after in vivo 5-bromo-2′-deoxyuridine labelling: a flow cytometric study of 75 tumours. British Journal of Surgery, 79, 335-339

    Google Scholar 

  • Shimomatsuya, T., Tanigawa, N. and Muraoka, R., 1991. Proliferation activity of human tumors: assessment using bromodeoxyuridine and flow cytometry. Journal of Cancer Research, 82, 357-362

    Google Scholar 

  • Steel, G.G., 1977. Growth Kinetics of Tumours: Cell Population Kinetics in Relation to the Growth and Treatment of Cancer, (Clarendon Press, Oxford) 246

    Google Scholar 

  • Schutte, B., Reynders, M.M.J., van Assche, C.L.M.V.J., Hupperets, P.S.G.J., Bosman, F.T. and Blijham, G.H., 1987. An improved method for the immunocytochemical detection of the bromodeoxyuridine labeled nuclei using flow cytometry. Cytometry, 8, 372-376

    Google Scholar 

  • Terry, N.H.A., White, R.A., Meistrich, M.L. and Calkins, D.P., 1991. Evaluation of flow cytometric methods for determining population potential doubling times using cultured cells. Cytometry, 12, 234- 241

    Google Scholar 

  • Terry, N.H.A., White, R.A. and Meistrich, M.L., 1992. Cell kinetics: from tritiated thymidine to flow cytometry. British Journal of Radiology, 24, 153-157

    Google Scholar 

  • Vail, D.M., 1993. Analysis of tumor cell-cycle kinetics: a tool for predicting prognosis and treatment response. Proceedings of the 11th ACVIMForum, 778–781

  • van Erp, P.E.J., Brons, P.P.T., Boezeman, J.B.M., de Jongh, G.J. and Bauer, F.W., 1988. A rapid flow cytometric method for bivariate bromodeoxyuridine/DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation. Cytometry, 9, 627-630

    Google Scholar 

  • Vindeløv, L.L. and Christensen, I.J., 1990. A review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. Cytometry, 11, 753-770

    Google Scholar 

  • White, R.A., Terry, N.H.A., Meistrich, M.L. and Calkins, D.P., 1990. Improved method for computing potential doubling time from flow cytometric data. Cytometry, 11 314-317

    Google Scholar 

  • Wilson, G.D., McNally, N.J., Dunphy, E., Karcher, H. and Pfagner, R., 1985. The labelling index of human and mouse tumors assessed by bromodeoxyuridine staining in vitro and in vivo and flow cytometry. Cytometry, 6, 641-647

    Google Scholar 

  • Wilson, G.D., McNally, N.J., Dische, S. and Bennett, M.H., 1988a. Cell proliferation in human tumours measured by in-vivo labelling with bromodeoxyuridine. British Journal of Radiology, 61, 419-422

    Google Scholar 

  • Wilson, G.D., McNally, N.J., Dische, S., Saunders, M.I., Des Rochers, C., Lewis, A.A. and Bennett, M.H., 1988b. Measurement of cell kinetics in human tumours in vivo using bromodeoxyuridine incorporation and flow cytometry. British Journal of Cancer, 58, 423-431

    Google Scholar 

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Authors and Affiliations

  1. Veterinary Medicine Department, Radio-Oncology Programme, University of Zürich, Winterthurerstr. 260, CH-8057, Zürich, Switzerland

    U. Schwyn, B. Klink, A. Parvis, D. Ruslander & B. Kaser-Hotz

  2. Institute of Veterinary Pathology, University of Zürich, Winterthurerstr. 260, CH-8057, Zürich, Switzerland

    B. Hauser

  3. Paul Scherrer Institute, CH-5232, Villigen, Switzerland

    N.E.A. Crompton & H. Blattmann

Authors
  1. U. Schwyn
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  2. N.E.A. Crompton
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  3. H. Blattmann
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  4. B. Hauser
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  5. B. Klink
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  6. A. Parvis
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  7. D. Ruslander
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  8. B. Kaser-Hotz
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Schwyn, U., Crompton, N., Blattmann, H. et al. Potential tumour doubling time: determination of Tpot for various canine and feline tumours. Vet Res Commun 22, 233–247 (1998). https://doi.org/10.1023/A:1006087114421

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