Abstract
A method for the determination of cross sections for gas-phase protein ions, based on the energy loss of ions as they pass through a collision gas, is described. A simple model relates the energy loss to the number of collisions and hence the cross section. Results from a Monte Carlo model that support the validity of this approach are described. Experimental cross sections are reported for motilin, ubiquitin, cytochrome c, myoglobm, and bovine serum albumin. Cross sections range from approximately 800 Å2 for motilin to approximately 14,000 Å2 for bovine serum albumin and generally increase with the number of charges on the ion. Cytochrome c ions from aqueous solution show somewhat smaller cross sections than ions formed from solutions of higher organic content, suggesting that the gas-phase ions may retain some memory of their solution conformation.
Article PDF
Similar content being viewed by others
References
Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Science, 1989, 246, 64.
Smith, R. D.; Barinaga, C. J. Rapid Commun. Mass Spectrom. 1990, 4, 54.
Douglas, D. J.; French, J. B. J. Am. Soc. Mass Spectrom. 1991, 3, 398.
Bohr, N. Philos. Mag. 1913, 25, 10.
Douglas, D. J. J. Phys. Chem. 1982, 56, 185–191.
Kauzmann, W. Kinetic Theory of Gases; W. A. Benjamin: New York, 1966.
Covey, T. R.; Bonner, R. F.; Shushan, B. I. Rapid Commun. Mass Spectrom. 1988, 2, 249.
Chowdury, S. K.; Katta, V.; Chait, B. J. J. Am Chem. Soc. 1990, 112, 9012.
Loo, J.A.; Loo, R. R. O.; Usdeth, H. R.; Edmonds, C. G.; Smith, R. D. Rapid Commun. Mass Spectrum. 1991, 5, 101.
McDaniel E. W. Collision Phenmena in Ionized Gases; John Wiley: New York, 1964, p. 72.
Gombas, P. In Handbuch der Physik, vol. 36; Flugge, S. Ed., Springer-Verlag; Berlin 1956; p. 192.
Feng, R.; Konish Y. Proceedings of the 40th ASMS Conference on Mass Spectrometry and Allied Topics; Washington, DC, May 31–June 5, 1992.
Suckau, D.; Shi, Y.; Quinn, J. P.; Senko, M. W.; Zhang, M.; McLafferty, F. W. Proceedings of the 40th ASMS Conference on Mass Spectrometry and Allied Topics; Washington, DC, May. 31–June 5, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Covey, T., Douglas, D.J. Collision cross sections for protein ions. J Am Soc Mass Spectrom 4, 616–623 (1993). https://doi.org/10.1016/1044-0305(93)85025-S
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/1044-0305(93)85025-S