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Ovarian Rejuvenation Through Platelet-Rich Autologous Plasma (PRP)—a Chance to Have a Baby Without Donor Eggs, Improving the Life Quality of Women Suffering from Early Menopause Without Synthetic Hormonal Treatment

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Abstract

Due to the fact that modern American and European women postpone childbirth until later in life, they are more likely to face the problem of ovarian insufficiency by the time they are ready to have children. So, the ability to restore the ovarian function safely is crucially important. Our study involved 38 women 31–45 years of age with low ovarian reserves and at least two unsuccessful attempts to receive their oocytes through IVF. The blood from the patients was collected into two BD vacutainers for PRP preparation. The platelet concentration in the PRP was 1 × 106 μl. PRP injections into the ovaries were performed by a gynecologist with a special 25G needle, 20 cm in length, as an ultrasound-guided procedure or a laparoscopic-assisted approach. After PRP treatment, women were tested with several criteria for 12 months. We saw a significant improvement in hormone levels; six healthy babies were born, ten pregnancies were achieved, and four out of the ten were from natural conception. The PRP injections into the ovaries are safe, productive, and a natural treatment that may help women with premature ovarian insufficiency to give birth to their own child. The difference and novelty with our method of ovarian rejuvenation is in obtaining a higher platelet concentration (about 1 × 106 μl), which allows us to achieve long-lasting results, within 12 months, after a single procedure. Additionally, for the first time, we proposed and successfully performed a laparoscopically assisted technique for administering PRP into the ovary.

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Abbreviations

PRP:

Platelet-rich autologous plasma

FSH:

Follicle-stimulating hormone

LH:

Luteinizing hormone

AMH:

Anti-Müllerian hormone

TNF:

Tumor necrosis factor

TGF:

Transforming growth factor

BMP:

Bone morphogenic protein

FSHR:

Follicle-stimulating hormone receptor

IGF:

Insulin-like growth factor

POF:

Premature ovarian failure

GF:

Growth factor

PDGF:

Platelet-derived growth factor

PDAF:

Platelet-derived angiogenesis factor

PF:

Platelet factor

EGF:

Epidermal growth factor

ECGF:

Epithelial cell grow factor

VEGF:

Vascular endothelial cell growth factor

bFGF:

Fibroblast growth factor

GSC:

Germline stem cell

IVF:

In vitro fertilization

hCG:

Human chorionic gonadotropin

SVF:

Stromal vascular fraction

MSC:

Mesenchymal stem/stromal cell

ADSC:

Adipose-derived stem/stromal cell

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Authors and Affiliations

  1. Ultrasound Pro Medical Centre, Kiev, Ukraine

    Nataliia Petryk & Mykhailo Petryk

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  1. Nataliia Petryk
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  2. Mykhailo Petryk
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Both authors contributed equally to this work.

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Correspondence to Nataliia Petryk.

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Competing Interests

The authors both hold the UA patent for the process and treatment using PRP injections into the ovaries.

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Electronic supplementary material

Ultrasound-guided ovarian rejuvenation procedure. Now you can see the ovary. The needle is going through the vaginal wall, puncturing the ovarian cortex. Enriched plasma is coming into the ovary. (MP4 35,030 kb).

PRP into the ovary with the laparoscopic approach. The needle is going through the abdominal wall, puncturing the ovarian cortex. Enriched plasma is coming into the ovary. (MP4 87,155 kb).

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Petryk, N., Petryk, M. Ovarian Rejuvenation Through Platelet-Rich Autologous Plasma (PRP)—a Chance to Have a Baby Without Donor Eggs, Improving the Life Quality of Women Suffering from Early Menopause Without Synthetic Hormonal Treatment. Reprod. Sci. 27, 1975–1982 (2020). https://doi.org/10.1007/s43032-020-00266-8

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  • DOI: https://doi.org/10.1007/s43032-020-00266-8

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