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Construction of DNA-based logic gates on nanostructured microelectrodes

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Abstract

Electrochemical logical operations utilizing biological molecules (protein or DNA), which can be used in disease diagnostics and bio-computing, have attracted great research interest. However, the existing logic operations, being realized on macroscopic electrode, are not suitable for implantable logic devices. Here, we demonstrate DNA-based logic gates with electrochemical signal as output combined with gold flower microelectrodes. The designed logic gates are of fast response, enzyme-free, and micrometer scale. They perform well in either pure solution or complex matrices, such as fetal bovine serum, suggesting great potential for in vivo applications.

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Acknowledgements

Ali Aldalbahi acknowledges the support by the Deanship of Scientific Research, College of Science Research Center at King Saud University.

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    Authors and Affiliations

    1. Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China

      Tao Wei & Yun Zhao

    2. Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China

      Tao Wei, Min Li, Yue-Yue Zhang, Li-Hua Wang & Xiao-Lei Zuo

    3. Chemistry Department, King Saud University, Riyadh, 11451, Saudi Arabia

      Ali Aldalbahi

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    1. Tao Wei
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    Correspondence to Li-Hua Wang, Xiao-Lei Zuo or Yun Zhao.

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    This work was supported by the National Natural Science Foundation of China (Nos. 31470960 and 21422508).

    Authors Tao Wei and Min Li contributed equally to this work.

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    Wei, T., Li, M., Zhang, YY. et al. Construction of DNA-based logic gates on nanostructured microelectrodes. NUCL SCI TECH 28, 35 (2017). https://doi.org/10.1007/s41365-017-0191-1

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