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Serum biomarkers in uncontrolled no heart-beating donors may identify kidneys that will never work after transplantation

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Journal of Nephrology Aims and scope Submit manuscript

Abstract

Background/aims

Kidneys from uncontrolled non heart-beating donors achieve a good level of renal function after transplantation. However, a number of them will never function in the recipient. Our aim was to determine if serum biomarkers associated with platelet activity, inflammation and the nitric oxide system in uncontrolled non heart-beating donors may help to predict no renal function recovery after renal transplantation.

Methods

Serum levels of interleukin (IL)-6, IL-10, intercellular cell adhesion molecule-1 (ICAM-1), cyclic guanosine monophosphate (cGMP), nitrite + nitrate and platelet factor-4 (PF4) were measured using enzyme-linked immunosorbent assay (ELISA) kits in 88 uncontrolled non heart-beating donors divided according to the renal functionality achieved in the recipients into functional (n = 76) and non functional (n = 12).

Results

Kidneys from donors with higher IL-6 levels (>900 pg/ml) were functional after transplantation. Serum cGMP levels below 372.3 fmol/l were also associated with kidneys that recovered the renal function. However, serum levels of PF4 showed the best correlation with recovery of renal functional in the recipients since they were significantly lower in the donors whose kidneys functioned after transplantation.

Conclusions

Serum PF4 levels in uncontrolled non heart-beating donors may be a good predictor for kidneys that never will reach functional recovery. Some serum cGMP, IL-6 and IL-10 levels may simply help identify kidneys that will function after transplantation.

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Acknowledgments

This work was supported by Fondo de Investigaciones de la Seguridad Social [Redes Temáticas de Investigación Cooperativa (RETICs) RD12/0042/0040], Fondo Europeo de Desarrollo Regional (Fondos FEDER). Javier Modrego is staff member of RIC, José J. Zamorano León is staff member of Comunidad de Madrid (S2010/BMD-2374). We thank Begoña Larrea for secretarial assistance.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical approval

The study was approved by the local Ethical committee in accordance with the ethical guidelines of the 2000 Declaration of Helsinki and 2008 Declaration of Istanbul.

Informed consent

Written consent was obtained for all 88 renal UCNHBD.

Author information

Author notes

    Authors and Affiliations

    1. Medicine Department, Universidad Complutense de Madrid, Madrid, Spain

      Antonio J. López-Farré

    2. Preventive Medicine and Public Health, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain

      Juana María Santos-Sancho

    3. Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain

      Javier Modrego, José J. Zamorano-León & Pablo Rodríguez-Sierra

    4. Health Sciences Institute, Talavera de la Reina, Toledo, Spain

      Antonio Segura

    5. Nephrology Department, Hospital Clínico San Carlos, Universidad Complutense de Madrid, C/Profesor Martín Lagos s/n, Madrid, 28040, Spain

      Leyre Martín, Ana Sánchez-Fructuoso, José Herrero & Alberto Barrientos

    6. Servicio de Atención Médica Urgente (SAMUR)-Protección Civil, Madrid, Spain

      Fernando Prados

    7. Servicio de Urgencia Médica de Madrid (SUMMA 112), Madrid, Spain

      Alonso Mateos

    8. Transplant Coordination Unit, Hospital Clínico San Carlos, Medicine Department, School of Medicine, Universidad Complutense de Madrid, C/Profesor Martín Lagos s/n, Madrid, 28040, Spain

      Francisco del Río

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    1. Antonio J. López-Farré
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    Correspondence to Alberto Barrientos.

    Additional information

    A. J. López-Farré, J. M. Santos-Sancho and J. Modrego have contributed by equal in the manuscript.

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    López-Farré, A.J., Santos-Sancho, J.M., Modrego, J. et al. Serum biomarkers in uncontrolled no heart-beating donors may identify kidneys that will never work after transplantation. J Nephrol 29, 119–127 (2016). https://doi.org/10.1007/s40620-015-0203-3

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