Abstract
Proteolysis is a form of the biological control providing fast physiological response to changing conditions of an environment. The goal of the present work was to analyze the localization of the Arg-X proteolysis in complexes of histones and non-histone proteins isolated from supra-molecular structures (nucleoplasm, chromatin, the nuclear matrix) of cell nuclei of mature wheat germs during induction of growth. Cell nuclei were isolated from germs, cleared, and then nucleoplasm, chromatin, nuclear matrix were extracted by increasing ionic strength of solution. From isolated supra-molecular structures, non-histone proteins were separated from histones by using ion exchange chromatography. The Arg-X proteolytic activity was assessed by cleavage of Arg-X bonds in the arginine-enriched protein protamine in all nuclear fractions. It was shown that in the coleoptile, which grows by cell elongation, Arg-X proteolysis activity was at the level of nuclear matrix structures, namely the linker histone HI and core histones, and in mesocotyls, Arg-X proteolysis activity was found in the core histones of the fraction of chromatin tightly bound to the nuclear matrix. A possible role of Arg-X proteolysis in the fine regulation of morphogenetic mechanisms occurring during seed germination is suggested.
References
Akberdin, I. R., Kazantsev, V. V., Sazonov, E. A., Mironova, V. V., Omeljanchuk, N. A., Gainova, I. A., Fadeev, S. I., Korolev, V. K., Medvedev, A. E., & Likhoshvai, V. A. (2011). Mathematical modeling of the shoot meristem at various hierarchical levels. Live Science, 3, 12–13. http://www.bionet.nsc.ru/live-journal/assets/files/journal2/007.pdf. Accessed 09 March 2016 (in Russian).
Danovich, K. N., Sobolev, A. M., Zhdanova, L. P., Illy, I. E., Nikolaev, M. G., Askochenskaya, N. A., et al. (1982). Physiology of seeds. Moscow: Nauka. (in Russian).
Ivanov, V. B. (1974). Cellular bases of plant growth. Moscow: Nauka. (in Russian).
Ivanov, V. B., Dobrochaev, A. E., & Baskin, T. I. (2002). What the distribution of cell lengths in the root meristem does and does not reveal about cell division. Journal of Plant Growth Regulation, 21, 60–67.
Ivanova, E. A., & Vafina, G. H. (1991). Method of isolation of plant cell nucleus. RF Patent, 1701747 (in Russian).
Ivanova, E. A., & Vafina, G. H. (1992). Method of obtaining of nuclear fractions possessing proteinase and inhibition activity. RF Patent, 1733471 (in Russian).
Ivanova, E. A., & Vafina, G. H. (2011). Method of preparative isolation of basic proteins from suprastructures of cell nuclei of plants. RF Patent, 2408602 (in Russian).
Morozova, Z. A., & Murashov, V. V. (2009). The genus Triticum L. morphogenesis kinds of wheat. Moscow: Triada. (in Russian).
Pikaard, C. (2012). Reading the second code: mapping epigenomes to understand plant growth, development and adaptation to the environment. The Plant Cell, 24, 2257–2261.
Razin, S. V., Borunova, V. V., Iarovaia, O. V., & Vassetzky, Y. S. (2014). Nuclear matrix and structural and functional compartmentalization of the eucaryotic cell nucleus. Biochemistry (Mosc), 79(7), 608–618. doi:10.1134/S0006297914070037. (in Russian).
Skowronska-Krawczyk, D., & Rosenfeld, M. G. (2015). Nuclear matrix revisited? Cell Cycle, 14(10), 1487–1488.
Vossaert, L., Meert, P., Scheerlinck, E., Glibert, P., Van Roy, N., Heindryckx, B., et al. (2014). Identification of histone H3 clipping activity in human embryonic stem cells. Stem Cell Research, 13(1), 123–134. doi:10.1016/j.scr.2014.05.002.
Zhou, P., Wu, E., Alam, H. B., & Li, Y. (2014). Histone cleavage as a mechanism for epigenetic regulation: current insights and perspectives. Current Molecular Medicine, 14(9), 1164–1172.
Zubairova, Y. S., & Nikolaev, S. V. (2014). Models of regulation of stem cell niche structure in shoot apical meristem. Russian Journal of Genetics: Applied Research, 17(4/1), 738–747. (in Russian).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Vafina, G.H., Ivanov, R.S. Localization of Arg-X proteolysis in the supramolecular structures of cell nuclei during the induction of growth in mature wheat germs. Ind J Plant Physiol. 21, 370–373 (2016). https://doi.org/10.1007/s40502-016-0235-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40502-016-0235-2