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Effects of soil pH and arbuscular mycorrhiza (AM) inoculation on growth and chemical composition of chia (Salvia hispanica L.) leaves

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Abstract

In this study, chemical composition and growth responses of chia plants (Salvia hispanica L.) to inoculation with an arbuscular mycorrhiza (AM, Glomus mosseae, Nicol. & Gerd.) fungal inoculum (namely MC10) under the influence of soil pH were investigated. The experiment project included six treatments, i.e., control-non-arbuscular mycorrhiza fungi (NAMF, pH 7.1), control-arbuscular mycorrhiza fungi (AMF, pH 7.1), acid-NAMF (pH 5.1), acid-AMF (pH 5.1), alkaline-NAMF (pH 8.2), and alkaline-AMF (pH 8.2). Stunted growth and leaf chlorosis were noticed mainly in plants grown in soil with acidic pH. An increase in fresh biomass was attained in plants amended with AM fungi in alkaline soil pH. Alkaline sandy soil with low levels of available P stimulated AMF colonization of chia roots, which subsequently enhanced P uptake and translocation in plant tissues. Total proteins, carbohydrates, and total fat content in leaves increased in AMF-inoculated plants in neutral and alkaline soil pH, while only fat content enhanced under acidic soil pH. MC10 inoculum resulted in reduced levels of total phenolics under alkaline conditions, whereas under acidic soil resulted in increased levels compared to the non-inoculated plants. The predominant fatty acids of chia leaves were palmitic (18.3 %), a-linolenic (17.1 %), pentadecenoic (11.0 %), linoleic (7.5 %), oleic (7.5 %), and stearic (6.3 %). Higher concentration of stearic, oleic, linoleic, and a-linolenic acids was observed in the leaves of chia plants grown on control (neutral pH) and alkaline soil in the presence of the MC10 inoculum. Alkaline soil combined with AM inoculation enhanced the nutritional value of chia leaves.

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Authors and Affiliations

  1. Institute of Food Technology, Hellenic Agricultural Organization-Demeter, 1 S. Venizelou str., 14123, Lycovrissi, Greece

    Georgia Ouzounidou & Eleftherios Eleftheriadis

  2. Laboratory of Plant & Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 &Aeolou, 41221, Larissa, Greece

    Vasiliki Skiada & Kalliope K. Papadopoulou

  3. Fisheries Research Institute, Hellenic Agricultural Organization-Demeter, N. Peramos, 64007, Kavala, Greece

    Nikolaos Stamatis

  4. Soil Science Institute of Athens, Hellenic Agricultural Organization-Demeter, 1 S. Venizelou str., 14123, Lycovrissi, Greece

    Victor Kavvadias

  5. Laboratory of Attica, Directorate of Laboratory Control, Hellenic Food Authority (EFET), 31 Anagenisseos & Serron, 14451, Nea Philadelphia, Greece

    Fragiskos Gaitis

Authors
  1. Georgia Ouzounidou
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  2. Vasiliki Skiada
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  3. Kalliope K. Papadopoulou
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  4. Nikolaos Stamatis
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  5. Victor Kavvadias
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  6. Eleftherios Eleftheriadis
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  7. Fragiskos Gaitis
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Correspondence to Georgia Ouzounidou.

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Ouzounidou, G., Skiada, V., Papadopoulou, K.K. et al. Effects of soil pH and arbuscular mycorrhiza (AM) inoculation on growth and chemical composition of chia (Salvia hispanica L.) leaves. Braz. J. Bot 38, 487–495 (2015). https://doi.org/10.1007/s40415-015-0166-6

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  • DOI: https://doi.org/10.1007/s40415-015-0166-6

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