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Effect of dentifrice of varying fluoride concentration on surface microhardness of fluorosed enamel: an in vitro study

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Abstract

Aim

To evaluate the effect of dentifrice with varying concentrations of fluoride on surface microhardness of fluorosed enamel as compared to a non-fluoridated dentifrice.

Study design

In vitro, comparative type.

Method

60 enamel blocks with score-3 enamel fluorosis were randomly divided into three groups: Group I non-fluoridated dentifrice, Group II 1000-ppm fluoridated dentifrice and Group III 1400-ppm fluoridated dentifrice. After evaluating the baseline surface microhardness, samples from each group were brushed for 2 min, twice daily for a period of 21 days with 0.02 g of respective interventional agents as per protocol. Microhardness of the samples was re-evaluated at the end of 21 days. Statistical analysis was performed using paired t test, ANOVA and Kruskal–Wallis test.

Results

The surface microhardness within the group from the baseline to 21st day post intervention, showed a statistically significant increase in all three groups, hence indicating that all the three interventional agents used were effective against the fluorosed enamel surface. Intergroup comparison of the surface microhardness of fluorosed enamel from baseline to 21st day post intervention showed a statistically significant difference (p < 0.001) amongst the groups with Group III proving most effective followed by Group I and Group II.

Conclusion

1400-ppm fluoridated dentifrice is more effective as compared to 1000-ppm fluoridated dentifrice and non-fluoridated dentifrice in increasing the surface microhardness of fluorosed enamel.

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References

  • Adriana FL. Effect of fluoridated dentifrice and acidulated phosphate fluoride application on early artificial carious lesions. Am J Dent. 2003;16(2):91–5.

    Google Scholar 

  • Ammari AB, Bloch-Zupan A, Ashley PF. Systematic review of studies comparing the anti-caries efficacy of children’s toothpaste containing 600 ppm of fluoride or less with high fluoride toothpastes of 1000 ppm or above. Caries Res. 2003;37(2):85–92.

    Article  PubMed  Google Scholar 

  • Biesbrock AR, Gerlach RW, Bollmer BW, et al. Relative anti-caries efficacy of 1100, 1700, 2200, and 2800 ppm fluoride ion in a sodium fluoride dentifrice over 1 year. Community Dent Oral Epidemiol. 2001;29(5):382–9.

    Article  PubMed  Google Scholar 

  • Chedid SJ, Cury JA. Effect of 0.02% NaF solution on enamel demineralization and fluoride uptake by deciduous teeth in vitro. Braz Oral Res. 2004;18:18–22.

    Article  PubMed  Google Scholar 

  • Chen Z, Luo Z, Yang S. Comparison of the effect of fluoride and non-fluoride dentifrices on enamel demineralization and remineralization in vitro. Hua Xi Kou Qiang Yi Xue Za Zhi. 2001;19(1):20–2.

    PubMed  Google Scholar 

  • Davies RM, Davies GM. High fluoride toothpastes: their potential role in a caries prevention programme. Dent Update. 2008;35(5):320–3.

    PubMed  Google Scholar 

  • Den Besten P, Li W. Chronic fluoride toxicity: dental fluorosis. Monogr Oral Sci. 2011;22:81–96.

    Article  Google Scholar 

  • Featherstone JDB. The science and practice of caries prevention. J Am Dent Assoc. 2000;131:887–99.

    Article  PubMed  Google Scholar 

  • Fejerskov O, Manji F, Baelum V. The nature and mechanisms of dental fluorosis in man. Dent. Res. 1990;69:692–700.

    Article  Google Scholar 

  • Fejerskov O, Yanagisawa T, Tohda H, et al. Posteruptive changes in human dental fluorosis: a histological and ultrastructural study. Proc. Finn. Dent. Soc. 1991;87:607–19.

    PubMed  Google Scholar 

  • Giambro NJ, Prostak K, Den Besten PK. Characterization of fluorosed human enamel by color reflectance, ultrastructure, and elemental composition. Caries Res. 1995;29:251–7.

    Article  PubMed  Google Scholar 

  • Hamba H, Nikaido T, Inoue G, Sadr A, Tagami J. Effects of CPP-ACP with sodium fluoride on inhibition of bovine enamel demineralization: a quantitative assessment using micro-computed tomography. J Dent. 2011;39(6):405–13.

    Article  PubMed  Google Scholar 

  • Jabbarifar SE, Salavati S, Nilchian F. Effect of fluoridated dentifrices on surface microhardness of the enamel of deciduous teeth. Dent Res J. 2011;8(3):113–7.

    Google Scholar 

  • Janavathi J, Rajnikanth R, Jyothi L, Kumar N, Rao TT. The effect of McInnes solution on enamel and the effect of GC tooth mousse on bleached enamel: an in vitro study. Indian J Mednodent Allied Sci. 2014;2(1):21–8.

    Article  Google Scholar 

  • Lata S, Varghese O, Varughese JM. Remineralization potential of fluoride and amorphous calcium phosphate-casein phospho peptide on enamel lesions: an in vitro comparative evaluation. Conserv Dent. 2010;13(1):42–6.

    Article  Google Scholar 

  • Marines NS, Lidiany KA, Altair A, et al. In situ effect of a dentifrice with low fluoride concentration and low pH on enamel remineralization and fluoride uptake. J Oral Sci. 2007;49(2):147–54.

    Article  Google Scholar 

  • Marks RG, Conti AJ, Moorhead JE, Cancro L, D’Agostino RB. Results from a three-year caries clinical trial comparing NaF and SMFP fluoride formulations. Int Dent J. 1994;44(3 Suppl 1):275–85.

    PubMed  Google Scholar 

  • Newbrun E. Studies on the physical properties of fluorosed enamel—II microhardness. Arch Oral Biol. 1960;2(1):21–7.

    Article  Google Scholar 

  • Reddy VVS, Indu Shekar VR. In-vitro evaluation of NaF and APF gel application on surface microhardness of enamel in children of optimum and high fluoride area. JISPPD. 1992;10:1–6.

    Google Scholar 

  • Reynolds EC, Cai F, Shen P, et al. Retention in plaque and remineralization of enamel lesions by various forms of calcium in a mouth rinse or sugar-free chewing gum. J Dent Res. 2003;82(3):206–11.

    Article  PubMed  Google Scholar 

  • Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and casein phosphopeptide–amorphous calcium phosphate. J Dent Res. 2008;87(4):344–8.

    Article  PubMed  Google Scholar 

  • Sapov K, Gedalia I, Grobler S, et al. A laboratory assessment of enamel hypoplasia of teeth with varying severities of dental fluorosis. J Oral Rehabil. 1999;26(8):672–7.

    Article  PubMed  Google Scholar 

  • Sato Y, Sato T, Niwa M, Aoki H. Precipitation of octacalcium phosphates on artificial enamel in artificial saliva. J Mater Sci Mater Med. 2006;17:1173–7.

    Article  PubMed  Google Scholar 

  • Shekar C, Cheluvaiah MB, Namile D. Prevalence of dental caries and dental fluorosis among 12 and 15 years old school children in relation to fluoride concentration in drinking water in an endemic fluoride belt of Andhra Pradesh. Indian J Public Health. 2012;56(2):122–8.

    Article  PubMed  Google Scholar 

  • Shen P, Manton DJ, Cochrane NJ. Effect of added calcium phosphate on enamel remineralization by fluoride in a randomized controlled in situ trial. J Dent. 2011;39(7):518–25.

    Article  PubMed  Google Scholar 

  • Shetty S, Hegde MN, Bopanna TP. Enamel remineralization assessment after treatment with three different remineralizing agents using surface microhardness: an in vitro study. J Conserv Dent. 2014;17:49–52.

    Article  PubMed  PubMed Central  Google Scholar 

  • Shinoda H. Effect of long-term administration of fluoride on physico-chemical properties of the rat incisor enamel. Calcif Tissue Res. 1975;18(1):91–100.

    Article  PubMed  Google Scholar 

  • Somani R, Jaidka S, Singh DJ, Arora V. Remineralizing potential of various agents on dental erosion. J Oral Biol Craniofacial Res. 2014;4(2):104–8. doi:10.1016/j.jobcr.2014.05.001.

    Article  Google Scholar 

  • Stookey GK, Mau MS, Isaacs RL, et al. The relative anti-caries effectiveness of three fluoride-containing dentifrices in Puerto Rico. Caries Res. 2004;38(6):542–50.

    Article  PubMed  Google Scholar 

  • Susheela AK. Fluorosis in developing countries: remedial measures and approaches. Proc Indian Natn Sci Acad. 2002;68:389–400.

    Google Scholar 

  • Thylstrup A. Posteruptive development of isolated and confluent pits in fluorosed enamel in a 6-year-old girl. Scand J Dent Res. 1983;91:243–6.

    PubMed  Google Scholar 

  • Vandana KL. Fluorosis and periodontium: a report of our institutional studies. J Int Clin Dent Res Organ. 2014;6(1):7–15.

    Article  Google Scholar 

  • Wolfgang AH, Andreas D, Stephanie L, et al. Effect of fluoride toothpastes on enamel demineralization. BMC Oral Health. 2006;6:8.

    Article  Google Scholar 

  • Yadav S, Khan TI, Gupta S, Gupta AB, Yadava RN. Fluorosis in India with special reference to Rajasthan. In: Proceedings of the international conference on water, environment, ecology, socioeconomics and health engineering (WEESHE), Seoul National University; 1999. p. 3–10.

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Authors and Affiliations

  1. Department of Pedodontics and Preventive Dentistry, JSS Dental College and Hospital, JSS University, Shivaratreshwar Nagar, Mysore, Karnataka, India

    R. Shanbhog, B. S. Nikitha & B. Nandlal

  2. Department of Community Dentistry, JSS Dental College and Hospital, JSS University, Mysore, Karnataka, India

    M. Thippeswamy

Authors
  1. R. Shanbhog
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  2. B. S. Nikitha
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  3. B. Nandlal
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  4. M. Thippeswamy
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Corresponding author

Correspondence to R. Shanbhog.

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This study was not funded.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Shanbhog, R., Nikitha, B.S., Nandlal, B. et al. Effect of dentifrice of varying fluoride concentration on surface microhardness of fluorosed enamel: an in vitro study. Eur Arch Paediatr Dent 17, 257–264 (2016). https://doi.org/10.1007/s40368-016-0237-9

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  • DOI: https://doi.org/10.1007/s40368-016-0237-9

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