Skip to main content
SpringerLink
Log in

Morphogenetic Studies on Two Mosses, Bryum dichotomum and Entodon macropodus Grown In Vitro

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Axenic cultures of two moss taxa viz. Bryum dichotomum Hedw., an acrocarpous moss and Entodon macropodus (Hedw.) Müll. Hal., a pleurocarpous moss were established by inoculating their spores into agar media. Germination, growth, development and comparative morphogenetic studies of these two growth forms were done. In B. dichotomum, protonemal buds developed on caulonema, while in E. macropodus, buds were produced on chloronema. Sub culturing of B. dichotomum gave rise to new individuals through chloronemal and caulonemal stage, while E. macropodus it directly regenerated into new gametophytes. In old culture under low moisture conditions, asexual diaspores namely, protonemal bulbils B. dichotomum and protonemal brood cells E. macropodus were formed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Sabovljevic A, Sabovljevic M, Jockovic N (2009) In vitro culture and secondary metabolite isolation in bryophytes. In: Jain SK, Saxena PK (eds) Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants. Humana Press, Springer, New York, pp 117–128

    Chapter  Google Scholar 

  2. Frank W, Decker EL, Reski R (2005) Physcomitrella patens is highly tolerant against drought, salt and osmotic stress. Planta 220:384–394

    Article  CAS  PubMed  Google Scholar 

  3. Decker EL, Reski R (2008) Current achievements in the production of complex biopharmaceuticals with moss bioreactors. Bioprocess Biosyst Eng 31:3–9

    Article  CAS  PubMed  Google Scholar 

  4. Cove DJ, Bezanilla M, Harris P, Quatrano R (2006) Mosses as model system for the study of metabolism and development. Ann Rev Plant Biol 57:497–520

    Article  CAS  Google Scholar 

  5. Duckett JG, Burch J, Fletcher PW, Matcham HW, Read DJ, Russell AJ, Pressel S (2004) In vitro cultivation of bryophytes: a review of practicalities, problems, progress and promise. J Bryol 26:3–20

    Google Scholar 

  6. Rowntree JK (2006) Development of novel methods for the initiation of in vitro bryophyte cultures for conservation. Plant Cell Tissue Organ Cult 87:191–201

    Article  Google Scholar 

  7. Awasthi V, Nath V, Asthana AK (2010) In vitro propagation of the endemic and threatened Indian liverwort: Cryptomitrium himalayense Kash. Curr Sci 98:1440–1441

    Google Scholar 

  8. Rowntree JK, Pressel S, Ramsay MM, Sabovljevic A, Sabovljevic M (2011) In vitro conservation of European bryophytes. In Vitro Cell Dev Biol Plant 47:55–64

    Article  Google Scholar 

  9. Awasthi V, Asthana AK, Nath V (2013) In vitro study on the reproductive behavior of the endemic and threatened Indian liverwort: Cryptomitrium himalayense Kash. Cryptogam Bryol 34:313–323

    Article  Google Scholar 

  10. Nishida Y (1978) Studies on sporeling types in mosses. J Hattori Bot Lab 44:371–454

    Google Scholar 

  11. Duckett JG, Schmid AM, Ligrone R (1998) Protonemal morphogenesis. In: Bates JW, Ashton NW, Duckett JG (eds) Bryology for the twenty first century. Maney Publishing and British Bryological Society, Leeds, pp 223–246

    Google Scholar 

  12. Duckett JG, Ligrone R (1992) A survey of diaspore liberation mechanism and germination pattern in mosses. J Bryol 17:335–354

    Article  Google Scholar 

  13. Sabovljevic M, Bijelovic A, Dragicevic I (2003) In vitro culture of mosses: Aloina aloides (K.F. Schultz) Kindb., Brachythecium velutimum (Hedw.) B.S.&G., Ceratodon purpureus (Hedw.) Brid., Eurhynchium praelongum (Hedw.) B.S. & G. and Grimmia pulvinata (Hedw.) Sm. Turk J Bot 27:441–446

    Google Scholar 

  14. Pressel S, Duckett JG (2009) Studies on protonemal morphogenesis in mosses. XII. Ephemeropsis, the xenith of morphological differentiation. J Bryol 31:67–75

    Article  Google Scholar 

  15. Awasthi V, Nath V, Asthana AK (2010) On the culture of a pleurocarpous moss Entodon laetus (Griff.) Jaeg. Natl Acad Sci Lett 33:145–148

    Google Scholar 

  16. Vujicic M, Sabovljevic A, Sabovljevic M (2011) Axenically culturing the bryophytes: establishment and propagation of the moss Hypnum cupressiforme (Bryophyta, Hypnaceae) in in vitro conditions. Bot Serbica 35:71–77

    Google Scholar 

  17. Awasthi V, Asthana AK, Nath V (2012) In vitro propagation of an epiphytic pleurocarpous moss Erythrodontium julaceum (Schwaegr.) Par. J Bryol 34:140–144

    Article  Google Scholar 

  18. Awasthi V, Nath V, Pande N, Asthana AK (2012) Morphogenetic study and in vitro propagation of two mosses: Philonotis thwaitesii Mitt. and Brachythecium plumosum (Hedw.). Taiwania 57:27–36

    Google Scholar 

  19. Knop W (1865) Quantitative Untersuchungen uber die Ernahrungsprozesse der Pflanzen. Landwirtseh Vers Stn 7:93–107

    Google Scholar 

  20. Kaul KN, Mitra GC, Tripathi BK (1962) Responses of Marchantia in aseptic culture to well known auxins and antiauxins. Ann Bot 26:447–466

    CAS  Google Scholar 

  21. Bopp M (1983) Developmental physiology of Bryophytes. In: Schuster RM (ed) New Manual of Bryology, vol 1. The Hattori Botanical Laboratory, Nichinan, pp 276–324

    Google Scholar 

  22. Nath V, Awasthi V, Asthana AK (2009) In vitro propagation and reproductive biology of moss Funaria hygrometrica Hedw. Int J Plant Reprod Biol 1:103–108

    Google Scholar 

  23. Sood S, Chopra RN (1973) A record preponment of bud induction in the moss Entodon myurus. J Plant Physiol 69:390–393

    CAS  Google Scholar 

  24. Andrew S, Redfearn PL (1965) Observations on the germination of the gemmae of Hyophila tortula (Schwaegr.) Hampe. Bryologist 68:345–347

    Article  Google Scholar 

  25. Vashistha BD, Chopra RN (1984) Production of protonemal gemmae in culture by the moss Didymodon recurvus (Griff.) Broth. New Phytol 97:83–86

    Article  Google Scholar 

  26. Pressel S, Matcham HW, Duckett JG (2007) Studies of protonemal morphogenesis in mosses. XI Bryum and allied genera: a plethora of propagules. J Bryol 29:241–258

    Article  Google Scholar 

  27. Spiess LD, Lippincot BB, Lippincot JA (1971) Development and gametophores initiation in the moss Pylaisiella selwynii as influenced by Agrobacterium tumefaciens. Am J Bot 58:726–731

    Article  Google Scholar 

  28. Salisbury FB, Ross CW (2002) Plant Physiology. CBS Publications and Distributors, New Delhi

    Google Scholar 

  29. Glime JM (2007) Ecophysiology of development: brood bodies. Bryophyt Ecol, Vol 1. Michigan Technological University and the International Association of Bryologists. http://www.bryoecol.mtu.edu. Accessed 6 Oct 2012

  30. Rowntree JK, Duckett JG, Mortimer CL, Ramsay MM, Pressel S (2007) Formation of specialized propagules resistant to desiccation and cryopreservation in the threatened moss Ditrichum plumbicola (Ditrichales, Bryopsida). Ann Bot 100:483–496

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

One of the authors (VA) is grateful to the University Grant Commission, New Delhi for providing the financial assistance under UGC-SAP (BSR) Research Fellowship Scheme.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Botany, D.S.B. Campus, Kumaun University, Nainital, 263002, India

    Vishal Awasthi, Anil Kumar Bisht & Neerja Pande

Authors
  1. Vishal Awasthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anil Kumar Bisht
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neerja Pande
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neerja Pande.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Awasthi, V., Bisht, A.K. & Pande, N. Morphogenetic Studies on Two Mosses, Bryum dichotomum and Entodon macropodus Grown In Vitro. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 421–427 (2016). https://doi.org/10.1007/s40011-014-0463-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-014-0463-z

Keywords

Search

Navigation