Abstract
Alkalophilic Paenibacillus polymyxa CKWX1, capable of producing xylanase was isolated from Dendrocalamus strictus decomposing wood samples. High level of xylanase produced by using easily available inexpensive agricultural waste residues as substrates such as wheat straw supported maximal xylanase activity, (1.41 IU/mL) followed by apple pomace, (1.22 IU/mL), Bombax ceiba wood dust, (0.64 IU/mL), Eucalyptus sp. wood dust (0.56 IU/mL) and Pinus roxburghii wood dust (0.33 IU/mL). The decomposing wood may provide excellent source for isolation of xylanolytic bacteria because of their varied physical and chemical conditions. The optimum pH and temperature for growth of this organism was 7.0–9.0 and 30–40 °C, respectively. On optimization of cultural conditions viz. 1.0 % inoculum size containing 80 × 108 colony forming unit (CFU)/mL, 1.0 % wheat straw, pH 7.0, temperature 35 °C, yeast extract 1.0 %, Tween 20 0.05 %, xylanase activity was increased by 2.6 folds. The novel alkaline xylanase was produced efficiently by alkalophilic P. polymyxa CKWX1. These results indicate the potential of P. polymyxa CKWX1 xylanase to be useful in pulp and paper industry.
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Walia, A., Mehta, P., Chauhan, A. et al. Production of Alkalophilic Xylanases by Paenibacillus polymyxa CKWX1 Isolated from Decomposing Wood. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 83, 215–223 (2013). https://doi.org/10.1007/s40011-012-0122-1
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DOI: https://doi.org/10.1007/s40011-012-0122-1