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Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity

  • Original Article
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency.

Methods:

We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM.

Results:

We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified.

Conclusion:

Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.

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Acknowledgements

This work was afforded by the National Natural Science Foundation of China (No. 81571824), Natural Science Foundation of Jiangsu Province (BK20141141) and Fundamental Research Funds for the Central Universities (lzujbky-2018-27). The authors thank Dr. Ma Hongwei of the division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences for the instruments and Materials required for the QCM assay. The mESCs were kindly provided by Dr. Tang Fuchou at Peking University.

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Authors and Affiliations

  1. Laboratory of Biomaterials and Regenerative Medicine, Academy for Advanced Interdisciplinary Studies, Peking University, No. 5 Yi-He-Yuan Road, Beijing, 100871, People’s Republic of China

    Siqi Zhang, Yan Li, Zuyuan Luo, Ping Zhou & Shicheng Wei

  2. School and Hospital of Stomatology, Xuzhou Medical University, No. 209 Tong-Shan Road, Xuzhou, 221004, People’s Republic of China

    Yuhua Sun

  3. Central Laboratory, Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, No. 22 Zhong-Guan-Cun South Road, Hai-Dian District, Beijing, 100081, People’s Republic of China

    Yi Sui, Xiao Xu & Shicheng Wei

  4. School and Hospital of Stomatology, Lanzhou University, No. 222 Tian-Shui South Road, Lanzhou, 730000, People’s Republic of China

    Ping Zhou

  5. National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University, No. 22 Zhong-Guan-Cun South Road, Hai-Dian District, Beijing, 100081, People’s Republic of China

    Shicheng Wei

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  1. Siqi Zhang
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  2. Yuhua Sun
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  3. Yi Sui
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  4. Yan Li
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  5. Zuyuan Luo
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  7. Ping Zhou
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Correspondence to Ping Zhou or Shicheng Wei.

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All the authors declared that they have no conflict of interest.

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There are no animal experiments carried out for this article.

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Zhang, S., Sun, Y., Sui, Y. et al. Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity. Tissue Eng Regen Med 15, 751–760 (2018). https://doi.org/10.1007/s13770-018-0138-6

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  • DOI: https://doi.org/10.1007/s13770-018-0138-6

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