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Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity

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An Erratum to this article was published on 10 April 2015

Abstract

Leaves from a natural population of Artemisia princeps var. orientalis (Pamp.) H. Hara were collected monthly from April through October and characterized for composition of secondary metabolite compounds and their phytotoxic effects on seed germination and seedling growth of Achyranthes japonica and Lactuca sativa. The compounds were identified using gas chromatography/mass spectrometry (GC/MS) coupled with a solvent-free solid injector (SFSI). GC/MS analyses of all samples revealed qualitative variability in the composition of secondary metabolites. The greatest number of compounds was identified in July (56) followed by September (30) and April (24), and the lowest number was found in June (2) and August (2). Among 92 compounds, the major compounds were various terpenes (23) (mono-, sesqui, di-, and tri-terpenes) followed by heterocyclic compounds (18) and hydrocarbons (14). The higher the concentration of the secondary metabolites, the lower the seed germination and seedling growth of A. japonica and L. sativa. Plant samples collected in July and August were most detrimental. Taken together, variability in the secondary metabolites compounds of A. princeps var. orientalis was verified during different seasons, and the compounds were successfully identified by a combination of SFSI and GC/MS. Notably, the antimicrobial and antioxidative effects were inconsistent throughout the various seasons.

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Acknowledgments

This study was supported by the MSIP (Ministry of Science, ICT and Future Planning).

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Authors and Affiliations

  1. Biotechnology Research Institute, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea

    M. I. R. Mamun, A. M. Abd El-Aty, Md. Musfiqur Rahman, Jeong-Heui Choi & Jae-Han Shim

  2. Department of Chemistry, University of Dhaka, Dhaka, 1000, Bangladesh

    M. I. R. Mamun

  3. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    A. M. Abd El-Aty

  4. Department of Oriental Medicine Resources, Suncheon National University, Suncheon, 540-742, Republic of Korea

    Kyeong Won Yun

  5. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Republic of Korea

    Ho-Chul Shin

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  1. M. I. R. Mamun
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  2. A. M. Abd El-Aty
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Correspondence to A. M. Abd El-Aty or Jae-Han Shim.

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Mamun, M.I.R., Abd El-Aty, A.M., Musfiqur Rahman, M. et al. Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity. J Korean Soc Appl Biol Chem 58, 173–183 (2015). https://doi.org/10.1007/s13765-015-0020-3

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