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Beneficial effects of the mixed adjuvant of CpG plus monophosphoryl lipid a in immunization with a recombinant protein vaccine for hepatitis A

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Abstract

In an effort to develop a new vaccine for hepatitis A, which is mainly transmitted via contaminated foods and water, recombinant virus protein 1 (VP1) of hepatitis A virus was used as an antigen. Several adjuvants in a single or a mixed form, i.e., alum, CpG oligodeoxynucleotide, monophosphoryl lipid A (MPL), alum plus MPL, and CpG plus MPL, were also tested for their immunological properties. When their effects on the production of VP1-specific IgG relative to that of total IgG and the levels of and balance between Th1- and Th2-type cytokine productions were compared, CpG plus MPL was found to have highly beneficial effects, providing a new insight in selection of adjuvant for development of a new vaccine.

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Authors and Affiliations

  1. Functional Materials Research Group., Korea Food Research Institute, 1201-62 AnyangPangyo-ro, Bundang-gu, Seongnam-si, 463-746, Republic of Korea

    Inkyu Hwang & Dong-Hwa Shon

  2. Functional Materials Research Group., Korea Food Research Institute, Seongnam, Republic of Korea

    Daewoon Choi & Hyejeong See

  3. Department of Microbiology, College of Medicine, Chuang-Ang University, Seoul, Republic of Korea

    Wonyong Kim

  4. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung-Hee University, Suwon, Republic of Korea

    In Sik Chung

Authors
  1. Inkyu Hwang
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  2. Daewoon Choi
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  3. Hyejeong See
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  4. Wonyong Kim
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  5. In Sik Chung
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  6. Dong-Hwa Shon
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Correspondence to Inkyu Hwang or Dong-Hwa Shon.

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Hwang, I., Choi, D., See, H. et al. Beneficial effects of the mixed adjuvant of CpG plus monophosphoryl lipid a in immunization with a recombinant protein vaccine for hepatitis A. J Korean Soc Appl Biol Chem 56, 95–98 (2013). https://doi.org/10.1007/s13765-012-2398-5

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  • DOI: https://doi.org/10.1007/s13765-012-2398-5

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