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Preparation of a cotton seed meal protein/nanoclay composite film containing carvacrol and its effect on the growth of escherichia coli O157:H7 inoculated on bacon during storage

  • Original Article
  • Food Science/Microbiology
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Abstract

Cottonseed meal protein (CSP) film containing nanoclay and carvacrol was prepared, and the physical properties of the film were investigated. Nanoclay (Cloisite Na+) incorporation improved mechanical properties of the CSP film. CSP film containing 3% nanoclay had tensile strength of 4.07 MPa and elongation at break of 26.37%. The optimal condition for the CSP film manufacturing was 3 g cottonseed protein, 3 g fructose, and 0.09 g Cloisite Na+ in 100 mL of film-forming solution. To inhibit the growth of pathogenic bacteria such as Escherichia coli O157:H7, CSP film containing carvacrol was prepared, and microbial growth was examined during bacon packaging and storage using the film. The antimicrobial activity of the CSP film against E. coli O157:H7 increased with increasing carvacrol concentration. After 10 d of storage, the population of E. coli O157:H7 inoculated on the bacon samples packed with the CSP film containing carvacrol decreased by 1.57 log CFU/g compared to the control. These results suggest that the CSP film containing carvacrol can be used as an antimicrobial packaging film.

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Authors and Affiliations

  1. Department of Food Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Wan-Shin Jo, Hye-Yeon Song, Nak-bum Song & Kyung Bin Song

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  1. Wan-Shin Jo
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  2. Hye-Yeon Song
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  3. Nak-bum Song
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  4. Kyung Bin Song
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Correspondence to Kyung Bin Song.

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Jo, WS., Song, HY., Song, Nb. et al. Preparation of a cotton seed meal protein/nanoclay composite film containing carvacrol and its effect on the growth of escherichia coli O157:H7 inoculated on bacon during storage. J Korean Soc Appl Biol Chem 55, 651–656 (2012). https://doi.org/10.1007/s13765-012-2100-y

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  • DOI: https://doi.org/10.1007/s13765-012-2100-y

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