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Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light

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Abstract

The potential eco-toxicity of fourteen different nanosized titanium dioxide (TiO2) particles was studied using Gram-positive Bacillus subtilis and Gram-negative Escherichia coli (ATCC K12) as test organisms. These photosensitive nanoparticles (NPs) were found to be harmful to the organisms studied at different degrees; the antibacterial activity increased with primary particle size, reached the maximum level in the range of 16–20 nm, and then decreased as the primary particle size increased. The presence of light played a significant role on the eco-toxicity of the nano-TiO2 particles under most conditions studied, presumably due to the generation of reactive oxygen species (ROS). However, bacterial growth was inhibited also under dark condition, indicating that mechanisms other than photocatalytic ROS were responsible for the toxic effect. Results highlight the need for caution during the use and disposal of manufactured NPs as to prevent unintended environmental impacts, as well as the importance of further research on the mechanisms and factors that control the toxicity of NPs toward aquatic organisms.

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Acknowledgments

The materials presented in this paper were based upon work supported by US-EPA under STAR Project Grant No. R-83172101. Dr. Nora Savage was the project manager of the above US-EPA project. We wish to show our gratitude to Professor Mao Hong Fan, Wyoming University, for providing us the nano-iron particles. The first author also wishes to thank YÖK (The Council of Turkish Higher Education) and Akdeniz University, Turkey, for the award of a graduate fellowship. Nanomaterials used in this study came from Sachtleben Chemicals, Nanostructured and Amorphous Materials, Inc., and Degussa Corp. Authors have no direct financial interest in or relationship with these companies. This manuscript has not been reviewed by the funding agency. All conclusions and interpretation presented in the paper were those of the authors’.

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Authors and Affiliations

  1. Department of Environmental Engineering, Akdeniz University, 07058, Antalya, Turkey

    A. Erdem

  2. Department of Civil and Environmental Engineering, University of Delaware, Newark, DE, 19716, USA

    A. Erdem, D. Metzler, D. Cha & C. P. Huang

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  1. A. Erdem
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  2. D. Metzler
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  3. D. Cha
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  4. C. P. Huang
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Correspondence to C. P. Huang.

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Erdem, A., Metzler, D., Cha, D. et al. Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light. Int. J. Environ. Sci. Technol. 12, 2987–2996 (2015). https://doi.org/10.1007/s13762-014-0729-2

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  • DOI: https://doi.org/10.1007/s13762-014-0729-2

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