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Polycarbonate biodegradation by isolated molds using clear-zone and atomic force microscopic methods

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Abstract

The accumulation of dry waste containing synthetic polymers due to their resistance to microorganisms and other environmental factors has posed some serious problems to the environment in recent years. On the other hand, plastics constitute the foundations of economy as they are widely used in agriculture, constructions, packaging, health care and also medicine. The aim of this research was to investigate the role of different isolated fungi in the degradation of polycarbonate polymers. For this purpose, sampling was done using the garden soil and waste leachate from Isfahan Waste Management Organization. Samples were enriched in the liquid mineral salt medium supplemented with polycarbonate and then were transferred to the same medium solidified with agar to isolate and identify different fungi. Finally, their biodegradation activity was investigated with the help of clear-zone and atomic force microscopic (AFM) techniques, and also lipase and amylase production was tested. Among 15 isolated genera of mold fungi, Fusarium, Ulocladium, Chrysosporium and Penicillium showed biodegradation activity. According to the diameter of clear zone around the fungal colonies and also AFM results, the highest rate of degradation was related to Fusarium. Lipase activity of all isolated fungi was positive, but amylase activity of Ulocladium was negative. It can be concluded that some fungal strains such as Fusarium can be used for the biodegradation of plastic materials as it leads to a very eco-friendly biodegradation process.

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References

  • Azevedo H, Reis R (2005) Understanding the enzymatic degradation of biodegradable polymers and strategies to control their degradation rate. CRC Press, LLC, Boca Raton, FL

    Google Scholar 

  • Behal A, Singh J, Sharma MK, Puri P, Batana N (2006) Characterization of alkaline α-amylase from Bacillus sp. AB 04. Int J Agric Biol 8(1):80–83

    CAS  Google Scholar 

  • Bentham RH, Morton LGH, Allen NG (1987) Rapid assessment of the microbial deterioration of polyurethanes. Int Biodeterior Biodegrad 23:377–386

    Article  CAS  Google Scholar 

  • Boyandin AN, Rudnev VP, Ivonin VN, Prudnikova SV, Korobikhina KI, Filipenko ML, Volova TG, Sinskey AJ (2012) Biodegradation of polyhydroxyalkanoate films in natural environments. Macromol Symp 320(1):38–42. doi:10.1002/masy.201251004

    Article  CAS  Google Scholar 

  • Chonde Sonal G, Chonde Sachin G, Bhosale PR, Nakade DB, Raut PD (2012) Studies on degradation of synthetic polymer Nylon 6 by fungus Trametes versicolor NCIM 1086. Int J Environ Sci 2(3):2435–2442

    Google Scholar 

  • Cosgrove L, Mc Geechan L, Robson GS, Handley P (2007) Fungal communities associated with degradation of polyester polyurethane in soil. App Environ Microbiol 73(18):5817–5824

    Article  CAS  Google Scholar 

  • Friedrich J, Zalar P, Mohorcic M, Klun U, Krzan A (2007) Ability of fungi to degrade synthetic polymer nylon-6. Chemosphere 67:2089–2095

    Article  CAS  Google Scholar 

  • Gilan I, Hadar Y, Sivan A (2004) Colonization, biofilm formation and biodegradation of polyethylene by a strain of R. ruber. Appl Microbiol Biotechnol 65:97–104

    CAS  Google Scholar 

  • Gupta VK, Mittal A, Malviya A, Mittal J (2009) Adsorption of carmoisine A from wastewater using waste materials—bottom ash and deoiled soya. J Colloid Interface Sci 335(1):24–33

    Article  CAS  Google Scholar 

  • Gupta VK, Rastogi A, Nayak A (2010) Biosorption of nickel onto treated alga (Oedogonium hatei): application of isotherm and kinetic models. J Colloid Interface Sci 342(2):533–539

    Article  CAS  Google Scholar 

  • Gupta VK, Agarwal Sh, Saleh AT (2011a) Chromium removal by combining the magnetic properties of iron oxide with adsorption properties of carbon nanotubes. Water Res 45(6):2207–2212

    Article  CAS  Google Scholar 

  • Gupta VK, Gupta B, Rastogi A, Agarwal S, Nayak A (2011b) A comparative investigation on adsorption performances of mesoporous activated carbon prepared from waste rubber tire and activated carbon for a hazardous azo dye—ACID Blue 113. J Hazard Mater 186(1):891–901

    Article  CAS  Google Scholar 

  • Jain AK, Gupta VK, Bhatnagar A (2003) A comparative study of adsorbents prepared from industrial wastes for removal of dyes. Sep Sci Technol 38(2):463–481

    Article  CAS  Google Scholar 

  • Kathirsan K (2003) Polythene and plastics-degrading microbes from the mangrove soil. Rev Biol Trop 51(3):629–634

    Google Scholar 

  • Kim DY, Rhee YH (2003) Biodegradation of microbial and synthetic polyesters by fungi. Appl Microbiol Biotechnol 61:300–308

    CAS  Google Scholar 

  • Kumaravel S, Hema R, Lakshmi R (2010) Production of polyhydroxybutyrate (Bioplastic) and its Biodegradation by Psudumonas Lemoiegenei and Aspergillus Niger. E. J Chem 7:536–542

    Google Scholar 

  • Lee KM, Gilmore DF, Huss MJ (2005) Fungal degradation of bioplastic PHD (Poly-3-hydroxy-butyric acid). J Polymers Environ 13(3):213–219

    Article  CAS  Google Scholar 

  • Leja K, Lewandowicz G (2009) Polymer biodegradation and biodegradable polymers—a review. Pol J Environ Stud 19:225–266

    Google Scholar 

  • Maeda H, Yamagata Y, Abe K, Hasegava F, Machida M, Ishioka R, Gomi K, Nakajima T (2005) Purification and characterization of biodegradable plastic-degrading enzyme from Aspergilus orizae. Appl Microbiol Biotechnol 67:78–788

    Article  Google Scholar 

  • Mittal A, Gupta VK, Malviya A, Mittal J (2008) Process development for the batch and bulk removal and recovery of a hazardous, water-soluble azo dye (Metanil Yellow) by adsorption over waste materials (Bottom Ash and De-Oiled Soya). J Hazard Mater 151(2–3):821–832

    Article  CAS  Google Scholar 

  • Mittal A, Mittal J, Malviya A, Gupta VK (2010) Removal and recovery of Chrysoidine Y from aqueous solutions by waste materials. J Colloid Interface Sci 344(2):497–507

    Article  CAS  Google Scholar 

  • Pranamuda H, Chollakup R, Tokiwa Y (1999) Degradation of polycarbonate by a polyester-degrading strain, Amycolatopsis sp. strain HT-6. Appl Environ Microbiol 65:4220–4222

    CAS  Google Scholar 

  • Premarj R, Mukesh D (2005) Biodegradation of polymers. Indian J Biotechnol 4:168–193

    Google Scholar 

  • Priyanka N, Archana T (2011) Biodegradability of polythene and plastic by the help of microorganism: a way for brighter future. Environ Anal Toxicol 1(4):1–4

    Google Scholar 

  • Reddy SV, Thriumala M, Mahmood SK (2008) Biodegradation of polyhydroxy alkanoates. Internet J Microbiol 4(2):95–99. doi:10.5580/1500

    Google Scholar 

  • Shah AA, Hasan F, Hameed A, Ahmad S (2007) Isolation and characterization of poly (3 hydroxybutyrate-co-3-hydroxyvalerate) degrading bacteria and purification of PHBV depolymerase from newly isolated. Int Biodeterior Biodegrad 60:109–115

    Google Scholar 

  • Shah AA, Hasan F, Hameed A, Ahmad S (2008) Biological degradation of plastics: a comprehensive review. Biotechnol Adv 26:246–265

    Article  CAS  Google Scholar 

  • Shrivastva N, Tripathi P (2011) Biodegradation of plastic polymer by selected fungal strains: a feasibility study. J Pharm Res 4(9):3056–3059

    Google Scholar 

  • Suyama T, Tokiwa Y (1997) Enzymatic degradation of aliphatic polycarbonate, poly (tetramethylene carbonate). Enzym Microb Technol 20:122–126

    Article  CAS  Google Scholar 

  • Suyama T, Hosoya H, Tokiwa Y (1998) Bacterial isolates degrading aliphatic polycarbonates. FEMS Microbiol Lett 161:255–261

    Article  CAS  Google Scholar 

  • Tokiwa Y, Suzuki T (1981) Hydrolysis of polyesters containing aromatic and aliphatic ester blocks by lipase. J Appl Polym Sci 26:441–448

    Article  CAS  Google Scholar 

  • Tokiwa Y, Calabia B, Ugwu C, Aiba S (2009) Biodegradability of plastics. Int J Mol Sci 10:3722–3742

    Article  CAS  Google Scholar 

  • Trishual A, Mukesh D (2008) Biodegradation of aliphatic and aromatic polycarbonates. Macromol Biosci 8:14–24

    Article  Google Scholar 

  • Trishual A, Mukesh D (2009) Fouling and degradation of polycarbonate in seawater: field and lab studies. J Polym Environ 17:170–180

    Article  Google Scholar 

  • Vijaya CH, Malikarjuna R (2008) Impact of soil composting using municipal solid waste on biodegradation of plastics. Indian J Biotechnol 7:235–239

    Google Scholar 

Download references

Acknowledgments

The authors wish to extend their sincere gratitude to all who assisted in promoting the present work, especially Isfahan Waste Management Organization.

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Authors and Affiliations

  1. Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran

    M. Arefian

  2. Khorasgan (Isfahan) Branch, Department of Basic Medical Sciences, Islamic Azad University, Isfahan, Iran

    M. Zia & A. Tahmourespour

  3. Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

    M. Bayat

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  1. M. Arefian
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  2. M. Zia
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  3. A. Tahmourespour
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  4. M. Bayat
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Correspondence to A. Tahmourespour.

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Arefian, M., Zia, M., Tahmourespour, A. et al. Polycarbonate biodegradation by isolated molds using clear-zone and atomic force microscopic methods. Int. J. Environ. Sci. Technol. 10, 1319–1324 (2013). https://doi.org/10.1007/s13762-013-0359-0

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  • DOI: https://doi.org/10.1007/s13762-013-0359-0

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