Skip to main content
SpringerLink
Log in

Biosensing of naringin in marketed fruits and juices based on its interaction with DNA

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

A new electrochemical biosensor is introduced to study naringin–DNA interaction and naringin detection, using a modified pencil graphite electrode (PGE). The biosensor was prepared by modification of a PGE with MWCNTs-poly dialyldimethylammonium chloride (PDDA) decorated with ds-DNA (dsDNA/PDDA-MWCNTs/PGE). Change in the oxidation signals of adenine and guanine was used as probes for the biosensor evaluation, using differential pulse voltammetry. Under the optimized conditions, naringin could be measure from 0.058 to 580.0 µg mL−1 with a limit of detection of 0.010 µg mL−1. The relative standard deviation for five measurements of 0.58 µg mL−1 naringin was found as 3.7 and 4.2 %, based on the guanine and adenine signals, respectively. The biosensor was used as a selective sensor for the determination of naringin. In addition, electrochemical impedance spectroscopy was performed to evaluate the charge-transfer resistance of the modified and unmodified PGEs. Finally, this highly stable electrochemical biosensor was used to analyze naringin contents in various citrus juices.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. K. Shetty, Process Biochem. 39, 789 (2004)

    Article  CAS  Google Scholar 

  2. J.B. Harborne, C.A. Williams, Phytochemistry 55, 481 (2000)

    Article  CAS  Google Scholar 

  3. G. Cao, E. Sofic, R.L. Prior, Free Radic. Biol. Med. 22, 749 (1997)

    Article  CAS  Google Scholar 

  4. K. Lemanska, H. Szymusiak, B. Tyrakowsla, R. Zielinski, A.E.M. Soffers, I.M.C.M. Rietens, Free Radic. Biol. Med. 31, 869 (2001)

    Article  CAS  Google Scholar 

  5. D.I. Kim, S.J. Lee, S.B. Lee, K. Park, W.J. Kim, S.K. Moon, Carcinogenesis 29, 1701 (2008)

    Article  CAS  Google Scholar 

  6. S. Kanno, A. Shouji, A. Tomizawa, T. Hiura, Y. Osanai, M. Ujibe, Life Sci. 78, 673 (2006)

    Article  CAS  Google Scholar 

  7. D. Singh, V. Chander, K. Chopra, Toxicology 201, 1 (2004)

    Article  CAS  Google Scholar 

  8. D.G. Guadagni, V.P. Maier, J.G. Turnbaugh, J. Sci. Food Agri. 24, 1277 (1973)

    Article  CAS  Google Scholar 

  9. A. Ghosal, H. Satoh, P.E. Thomas, F. Bush, D. Moore, Drug. Metab. Dispos. 24, 940 (1996)

    CAS  Google Scholar 

  10. K. Ishii, T. Furuta, Y. Kasuya, J. Agri. Food Chem. 48, 56 (2000)

    Article  CAS  Google Scholar 

  11. Y.S. Lee, M.M. Reidenberg, Pharmacology 56, 314 (1998)

    Article  CAS  Google Scholar 

  12. H. Terada, M. Miyabe, Food Hyg. Saf. Sci. 38, 219 (1997)

    Article  CAS  Google Scholar 

  13. K. Ishii, T. Furuta, Y. Kasuya, J. Chromatogr. B. 683, 225 (1996)

    Article  CAS  Google Scholar 

  14. W. Widmer, J. AOAC Inter. 83, 1155 (2000)

    CAS  Google Scholar 

  15. K. Ishii, T. Furuta, Y. Kasuya, J. Chromatogr. B. 704, 299 (1997)

    Article  CAS  Google Scholar 

  16. M. Careri, L. Elviri, A. Mangia, Rapid. Commun. Mass. Spectrom. 13, 2399 (1999)

    Article  CAS  Google Scholar 

  17. T.H. Tsai, J. Agri. Food. Chem. 50, 6669 (2002)

    Article  CAS  Google Scholar 

  18. M. Spanakis, S. Kasmas, I. Niopas, Biomed. Chromatogr. 23, 124 (2009)

    Article  CAS  Google Scholar 

  19. Y. Liu, F.G. Xu, Z.J. Zhang, R. Song, Y. Tian, Biomed. Chromatogr. 22, 736 (2008)

    Article  CAS  Google Scholar 

  20. Q. Yu, J. Xiang, W.F. Tang, M.Z. Liang, Y.P. Qin, F. Nan, J. Chromatogr. B. 877, 2025 (2009)

    Article  CAS  Google Scholar 

  21. F.G. Xu, Y. Liu, H.J. Dong, R. Song, Z.J. Zhang, Nat. Prod. Commun. 5, 795 (2010)

    CAS  Google Scholar 

  22. S.R. Mikkelsen, Electroanalysis 8, 15 (1996)

    Article  CAS  Google Scholar 

  23. E. Palek, M. Fojta, Anal. Chem. 73, 74A (2001)

    Article  Google Scholar 

  24. F. Lucarelli, G. Marrazza, A.P.F. Turner, M. Mascini, Biosens. Bioelectron. 19, 515 (2004)

    Article  CAS  Google Scholar 

  25. N. Sattarahmadi, Z. Kayani, H. Heli, Iranian. J. Chem. Soc. 12, 1569 (2015)

    Google Scholar 

  26. B. Habibi, M. Jahanbakhshi, Iranian. J. Chem. Soc. 12, 1431 (2015)

    CAS  Google Scholar 

  27. K. Xu, J. Huang, Z. Ye, Y. Ying, Y. Li, Sensors 9, 5534 (2009)

    Article  CAS  Google Scholar 

  28. A. Ozkan, Y. Sahin, Electroanalysis 21, 2363 (2009)

    Article  Google Scholar 

  29. N. Li, H.W. Zhao, R. Yuan, K.F. Peng, Y.Q. Chai, Electrochim. Acta. 54, 235 (2008)

    Article  CAS  Google Scholar 

  30. X.L. Li, H.B. Xiao, X.M. Liang, D.Z. Shi, J.G. Liu, J. Pharma. Biomed. Anal. 34, 159 (2004)

    Article  CAS  Google Scholar 

  31. A.A. Ensafi, M. Amini, B. Rezaei, Biosens. Bioelectron. 53, 43 (2014)

    Article  CAS  Google Scholar 

  32. A.A. Ensafi, E. Heydari-Bafrooei, B. Rezaei, Anal. Chem. 85, 991 (2013)

    Article  CAS  Google Scholar 

  33. G. Congur, A. Erdem, F. Mese, Bioelectrochemistry 102, 21 (2015)

    Article  CAS  Google Scholar 

  34. A. Özcan, Y. Sahin, M. Özsöz, S. Turan, Electroanalysis 19, 2208 (2007)

    Article  Google Scholar 

  35. E. Reichart, D. Obendorf, Anal. Chim. Acta. 360, 179 (1998)

    Article  CAS  Google Scholar 

  36. G.J. Volikakis, C.E. Efstathiou, Talanta 51, 775 (2000)

    Article  CAS  Google Scholar 

  37. A.R. Isabel, M.H.L. Ribeiro, Food Control 19, 432 (2008)

    Article  Google Scholar 

Download references

Acknowledgments

The authors wish to thank the Research Council of Isfahan University of Technology (IUT) and Center of Excellence in Sensor and Green Chemistry.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Isfahan University of Technology, Isfahan, 84156–83111, Iran

    Ali A. Ensafi, Sana Karbalaei & B. Rezaei

  2. Department of Chemistry, Faculty of Science, Vali-e-Asr University, Rafsanjan, P.O. Box 518, Iran

    Esmaeil Heydari-Bafrooei

Authors
  1. Ali A. Ensafi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sana Karbalaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Esmaeil Heydari-Bafrooei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Rezaei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ali A. Ensafi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ensafi, A.A., Karbalaei, S., Heydari-Bafrooei, E. et al. Biosensing of naringin in marketed fruits and juices based on its interaction with DNA. J IRAN CHEM SOC 13, 19–27 (2016). https://doi.org/10.1007/s13738-015-0707-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-015-0707-8

Keywords

Search

Navigation