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Binding preferential of chickpea cold shock protein during nucleic acid interactions

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Abstract

Cold shock proteins (CSPs) have a widespread occurrence from prokaryotes to eukaryotes including plants. These proteins are known to possess nucleic acid binding properties. CSPs have a single cold shock domain in prokaryotes while N-terminal and C-terminal flanking regions are present in eukaryotic CSPs. The objective of this study was to investigate nucleic acid binding preferential for the chickpea CSP. Full cDNA of chickpea CSP was cloned and sequenced. The sequence was submitted to GenBank (accession no. KM036036) at NCBI. Multiple sequence alignment and phylogenetic analysis further revealed that the inferred amino acid sequence belongs to CSP family. Molecular docking was performed between the CSP and variety of nucleic acids entities. These results suggest that CSPs of chickpea possess preferential binding affinity for single stranded nucleic acids. Docking results suggest that homo-polymer entities of RNA polyU RNA (20mer) form most stable complex.

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Acknowledgments

PB is thankful to MNNIT Allahabad for a M. Tech Fellowship. BSY is thankful to DST, New Delhi for PhD fellowship. SS is thankful to UGC New Delhi for a PhD fellowship. AM is thankful to TEQIP II for a project grant.

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Authors and Affiliations

  1. Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, 211004, India

    Pratibha Bhadauriya, Birendra Singh Yadav & Ashutosh Mani

  2. Center of Bioinformatics, University of Allahabad, Allahabad, 211002, India

    Swati Singh

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  1. Pratibha Bhadauriya
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  2. Birendra Singh Yadav
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  3. Swati Singh
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  4. Ashutosh Mani
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Correspondence to Ashutosh Mani.

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Bhadauriya, P., Yadav, B.S., Singh, S. et al. Binding preferential of chickpea cold shock protein during nucleic acid interactions. J. Plant Biochem. Biotechnol. 26, 208–215 (2017). https://doi.org/10.1007/s13562-016-0383-5

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