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Pulsed fed-batch strategy towards intensified process for lactic acid production using recycled paper sludge

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Abstract

A process has previously been optimized for production of lactic acid from recycled paper sludge (RPS), the ultimate solid waste obtained in the wastewater treatment plant of a local paper recycling mill. In this work, both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes were improved under batch mode in bench-scale bioreactor. The use of bioreactor was advantageous, providing higher conversion rates, together with increased yield. By running SSF in bioreactor, 73.2 g L−1 of lactic acid has been produced by Lactobacillus rhamnosus ATCC 7469 from 179 g L−1 RPS, corresponding to 76 % of maximum theoretical yield. Further process intensification, with improved kinetics and final product concentration, was achieved by applying a pulsed fed-batch strategy. With six pulsed additions of RPS (40 g at each 5 h), 108.2 g L−1 of lactic acid was produced (after 120 h, corresponding to 62 % yield). This achievement contributes to make more realistic the large-scale upgrading of RPS, a waste raw material exhibiting negative cost.

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Acknowledgments

This work was financially supported by EU INTEGRATED PROJECT Contract no. 026515-2. The authors gratefully acknowledge Dr. César Fonseca for the valuable discussions concerning the working plan and the critical reading of the manuscript and Eng. Raquel Pereira of Renova, S.A. (Torres Novas, Portugal), for providing the sludge material. The technical assistance of Carlos Barata is also acknowledged.

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Authors and Affiliations

  1. Laboratório Nacional de Energia e Geologia, I.P. (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038, Lisboa, Portugal

    S. Marques, F. M. Gírio & J. C. Roseiro

  2. Departamento de Bioengenharia, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisboa, Portugal

    J. A. L. Santos

  3. Institute for Bioengineering and Biosciences (IBB), Av. Rovisco Pais, 1049-001, Lisboa, Portugal

    J. A. L. Santos

Authors
  1. S. Marques
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  2. F. M. Gírio
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  3. J. A. L. Santos
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  4. J. C. Roseiro
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Corresponding author

Correspondence to S. Marques.

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Highlights

The process for RPS conversion into lactic acid was intensified at bioreactor scale.

RPS was effectively (76 % yield) converted into lactic acid (73 g L−1) by batch SSF.

Pulsed fed-batch strategy applied to SSF improved lactic acid production kinetics (108 g L−1, 62 % yield).

RPS upgrading will boost economic and environmental performance of paper industry.

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Marques, S., Gírio, F.M., Santos, J.A.L. et al. Pulsed fed-batch strategy towards intensified process for lactic acid production using recycled paper sludge. Biomass Conv. Bioref. 7, 127–137 (2017). https://doi.org/10.1007/s13399-016-0211-0

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  • DOI: https://doi.org/10.1007/s13399-016-0211-0

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