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para-Nitroaniline is a Promising Matrix for MALDI-MS Imaging on Intermediate Pressure MS Systems

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Journal of The American Society for Mass Spectrometry

Abstract

para-Nitroaniline (PNA) is presented as a promising matrix for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) on an intermediate-pressure ion source (~1 Torr) QqTOF instrument using an Nd:YVO4 laser operated at 5 kHz. An imaging study was carried out to determine the utility of PNA at this pressure by analyzing 14 tissue sections. We demonstrate acquisition of high-quality imaging data over a 6-h period in the ion source. In this study, comparisons were made between PNA and α-cyano-4-hydroxycinnamic acid (CHCA) in positive ion mode to demonstrate the utility of PNA in these circumstances. PNA performed as well as or better than CHCA in terms of lipid ion intensities, resulting in lower levels of ion fragmentation and in lower incidences of analyte migration at the edges of the tissue sections when using airspray matrix deposition.

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Acknowledgments

The authors thank the EPSRC for funding via studentships for R.T.S. and A.M.R. through the PSIBS Doctoral Training Centre at the University of Birmingham (EP/F50053X/1).

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Authors and Affiliations

  1. PSIBS Centre, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Rory T. Steven, Alan M. Race & Josephine Bunch

  2. School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Rory T. Steven, Alan M. Race & Josephine Bunch

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  1. Rory T. Steven
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  2. Alan M. Race
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Correspondence to Josephine Bunch.

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Steven, R.T., Race, A.M. & Bunch, J. para-Nitroaniline is a Promising Matrix for MALDI-MS Imaging on Intermediate Pressure MS Systems. J. Am. Soc. Mass Spectrom. 24, 801–804 (2013). https://doi.org/10.1007/s13361-013-0586-0

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  • DOI: https://doi.org/10.1007/s13361-013-0586-0

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