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Estimated time frame for successful PCR analysis of diamondback moths, Plutella xylostella (Lepidoptera: Plutellidae), collected from sticky traps in field conditions

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Abstract

PCR is a useful technique to detect mutations in insecticide resistance genes or to analyze gene flow in pest insects. Plutella xylostella (L.) is one of the most important pests that develops insecticide resistance, and it is desirable to make efficient use of specimens obtained from pheromone-baited sticky traps. To estimate the period of DNA preservation in moths collected on traps in the field, we analyzed the probability of PCR success using mtCOI and microsatellite primers. The results suggested that moths caught by sticky traps would maintain DNA of sufficient quality for PCR analysis when the trap was changed weekly. DNA degradation was greater in moth specimens on traps exposed to ultraviolet (UV) radiation in direct sunlight than in the shade. DNA degeneration was greater in moths exposed to direct sunlight in summer than in winter. Therefore, setting traps in shade or using a UV light-shielding barrier over the trap might be an effective approach to prevent DNA degradation.

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Acknowledgments

This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, PRM-4202). We thank Takeshi Shimoda for providing the laboratory-reared population of P. xylostella.

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Authors and Affiliations

  1. Plant Protection Division, Agricultural Research Center, The National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Ryuji Uesugi, Norihide Hinomoto & Chie Goto

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  1. Ryuji Uesugi
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  2. Norihide Hinomoto
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  3. Chie Goto
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Correspondence to Ryuji Uesugi.

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Uesugi, R., Hinomoto, N. & Goto, C. Estimated time frame for successful PCR analysis of diamondback moths, Plutella xylostella (Lepidoptera: Plutellidae), collected from sticky traps in field conditions. Appl Entomol Zool 51, 505–510 (2016). https://doi.org/10.1007/s13355-016-0418-3

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  • DOI: https://doi.org/10.1007/s13355-016-0418-3

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