Abstract
Superoxide dismutase (SOD) is an enzyme that catalyzes the dismutation of the superoxide anion. Homologs of a Cu/Zn-SOD gene were found in the genomes of most lepidopteran baculoviruses; however, its function is poorly understood. In this study, we characterized the sod gene of Bombyx mori (Lepidoptera: Bombycidae) nucleopolyhedrovirus (BmNPV). Western blot analysis revealed that His-tagged BmNPV SOD was expressed as an approximately 17-kDa protein. Reverse transcription-quantitative polymerase chain reaction demonstrated that BmNPV sod mRNA was detected at 8 h postinfection (hpi), peaked at 36 hpi, and declined gradually. In contrast, B. mori sod declined rapidly and was almost completely diminished from 36 hpi. To comprehend the role of BmNPV sod during virus infection, we used an sod-disrupted BmNPV bacmid. We found that deletion of sod from the BmNPV genome had little effect on virus propagation in cultured cells, whereas production of budded viruses and occlusion bodies were significantly delayed in B. mori larvae. These results indicate that BmNPV SOD plays a role in efficient virus propagation in B. mori larvae.
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Acknowledgments
We thank Dr. K. Iiyama (Kyushu University) for technical advice. We also thank Munetaka Kawamoto for clerical assistance. This work was supported by JSPS KAKENHI Grant Numbers 19688004 and 25292196 to S.K.
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Katsuma, S., Bando, H. & Shimada, T. Deletion analysis of a superoxide dismutase gene of Bombyx mori (Lepidoptera: Bombycidae) nucleopolyhedrovirus. Appl Entomol Zool 50, 57–62 (2015). https://doi.org/10.1007/s13355-014-0304-9
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DOI: https://doi.org/10.1007/s13355-014-0304-9