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Persian sturgeon insulin-like growth factor I: molecular cloning and expression during various nutritional conditions

  • Animal Genetics ∙ Original Paper
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Abstract

The effects of different periods of starvation (1, 2, 3, and 4 weeks) and subsequent re-feeding (over a 4 week) on the compensatory growth performance and insulin-like growth factor I (IGF-I) mRNA expression in liver and white muscle were investigated in juvenile Persian sturgeon (Acipenser persicus). First, a fragment of 617 nucleotides coding for IGF-I was cloned from liver, which included an open reading frame of 486 nucleotides, encoding a 162 amino acid preproIGF-I. This is composed of a 45 aa for signal peptide, a 117 aa for the mature peptide comprising the B, C, A, and D domains, and a 47 aa for E domain. The mature Persian sturgeon IGF-I exhibits high sequence identities with other sturgeon species and teleost, ranging between 68 and 95 %. The pattern of IGF-I mRNA expression in the liver and white muscle was measured in response to different periods of starvation and subsequent re-feeding. Nutritional status influenced IGF-I mRNA expression pattern in both liver and muscle. IGF-I mRNA expression in the liver increased during starvation, before decreasing after re-feeding. Furthermore, white muscle IGF-I mRNA expression showed better responses to nutritional status and decreased following starvation and increased by re-feeding. However, changes in the expression of IGF-I mRNA were not significantly different between any of the treatments in both tissues. These data suggest that muscle and liver IGF-I mRNA expression do not have a regulatory role for somatic growth induced by compensatory growth in Persain sturgeon.

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Acknowledgments

The research has been supported by Iranian Fisheries Research Organization and Agricultural Sciences and Natural Resources University of Gorgan and conducted in the International Sturgeon Research Institute. We thank the staff of International Sturgeon Research Institute and Shahid Beheshti Sturgeon Propagation Complex for their assistance during the experiment.

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Authors and Affiliations

  1. International Sturgeon Research Institute, PO Box: 41635-3464, Rasht, Iran

    Mahtab Yarmohammadi, Mohammad Pourkazemi, Rezvanollah Kazemi, Ali Hallajian & Mohammad Hassanzadeh Saber

  2. Gorgan University of Agricultural Sciences & Natural Resources, PO Box: 336, Gorgan, Iran

    Hassan Soltanloo

  3. Shahid Beheshti Rearing and Propagation Center, Rasht, Iran

    Alireza Abbasalizadeh

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  1. Mahtab Yarmohammadi
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  2. Mohammad Pourkazemi
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  3. Rezvanollah Kazemi
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  4. Ali Hallajian
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  5. Hassan Soltanloo
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  6. Mohammad Hassanzadeh Saber
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  7. Alireza Abbasalizadeh
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Correspondence to Mahtab Yarmohammadi.

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Yarmohammadi, M., Pourkazemi, M., Kazemi, R. et al. Persian sturgeon insulin-like growth factor I: molecular cloning and expression during various nutritional conditions. J Appl Genetics 55, 239–247 (2014). https://doi.org/10.1007/s13353-013-0192-7

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  • DOI: https://doi.org/10.1007/s13353-013-0192-7

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