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Characterization of influenza virus among influenza like illness cases in Mumbai, India

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Abstract

The present study was carried out to monitor influenza viruses by identifying the virus and studying the seasonal variation during 2007–2009 in Mumbai. A total of 193 clinical respiratory samples (nasal and throat swab) were collected from patients having influenza like illness in Mumbai region. One-step real-time reverse-transcriptase PCR (rRTPCR) was used to detect Influenza type A (H1 and H3) and Influenza type B virus. Isolation of the virus was carried out using in vitro system which was further confirmed and typed by hemagglutination assay and hemagglutination inhibition assay. Out of 193 samples 24 (12.4 3%) samples tested positive for influenza virus, of which 13 (6.73 %) were influenza type A virus and 10 (5.18 %) were influenza type B virus, while 1 sample (0.51 %) was positive for both. By culture methods, 3 (1.55 %) viral isolates were obtained. All the three isolates were found to be Influenza type B/Malaysia (Victoria lineage) by Hemagglutination Inhibition Assay. The data generated from the present study reveals that both Influenza type A and B are prevalent in Mumbai with considerable activity. The peak activity was observed during monsoon season.

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Acknowledgments

Authors thank the WHO Collaborating Center for Influenza, Centers for Disease Control and Prevention, Atlanta, Georgia, for providing the reagents and necessary protocols, and the Deans of B. J. Wadia Hospital for Children, Nair Hospital and Western railway unit hospital, Parel, Mumbai for the clinical samples. The Authors are also grateful to Dr. S. Muley, K. C. College, Mumbai for statistical analysis.

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Authors and Affiliations

  1. Department of Virology, Haffkine Institute, Aacharya donde Marg, Parel, Mumbai, India

    Soumen Roy, Ritwik Dahake, Deepak Patil, Shweta Tawde, Sandeepan Mukherjee, Shrikant Athlekar, Abhay Chowdhary & Ranjana Deshmukh

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  1. Soumen Roy
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  2. Ritwik Dahake
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  3. Deepak Patil
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  4. Shweta Tawde
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  5. Sandeepan Mukherjee
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  6. Shrikant Athlekar
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  7. Abhay Chowdhary
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  8. Ranjana Deshmukh
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Correspondence to Soumen Roy.

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Roy, S., Dahake, R., Patil, D. et al. Characterization of influenza virus among influenza like illness cases in Mumbai, India. VirusDis. 25, 372–375 (2014). https://doi.org/10.1007/s13337-013-0190-8

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  • DOI: https://doi.org/10.1007/s13337-013-0190-8

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