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Tissue distribution and ontogeny of multidrug resistance protein 2, a phosphatidylcholine translocator, in rats

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Abstract

Multidrug resistance protein 2 (Mdr2), encoded by ATP-binding cassette b4 (Abcb4), serves as a phospholipid flippase that is indispensable for phosphatidylcholine translocation. However, little was known about the regulation of Mdr2 in Sprague–Dawley rats, although they are commonly used for pre-clinical investigation as well as mechanistic study. Present study aims at determining the tissue distribution, gender difference and ontogeny of Mdr2 in rats on both gene and protein levels. Results showed that Mdr2 was highly expressed in liver, modestly enriched in brain and testis, and less distributed in gastrointestinal tracts. Gender-divergent and male-dominated distribution was observed in the Mdr2 mRNA expression of liver and generative organs. Developmental pattern of rat Mdr2 on protein level was not exactly consistent with that on mRNA level. In conclusion, there was a considerable distribution of rat Mdr2 in the brain, testis and intestine besides liver, and the ontogeny of Mdr2 performed in an age-dependent pattern with the post-transcriptional regulation.

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Fig. 1
Fig. 2

Abbreviations

MDR/Mdr:

Multidrug resistance protein

Abcb4:

ATP-binding cassette b4

PC:

Phosphatidylcholine

PCR:

Polymerase chain reaction

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GE:

Gene expression values

Ct:

Comparative threshold cycle

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Acknowledgments

This work was financially supported by Jiangsu province Nanjing City Innovative Graduate Research Program (No. CXZZ11_0828). We would like to acknowledge to Prof. Yubin Zhang and his lab members for their advice and support.

Conflict of interest

Qiuyang Zhang, Wei Yang, Hanlin Song, Hui Wu, Di Zhao, Yang Lu, Jiake He and Xijing Chen declare that they have no conflict of interest.

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Authors and Affiliations

  1. Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Mailbox 210, #24 Tongjiaxiang, Nanjing, 210009, Jiangsu, People’s Republic of China

    Qiuyang Zhang, Wei Yang, Hanlin Song, Hui Wu, Yang Lu, Jiake He, Di Zhao & Xijing Chen

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  1. Qiuyang Zhang
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  2. Wei Yang
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  3. Hanlin Song
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  4. Hui Wu
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  5. Yang Lu
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  6. Jiake He
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  7. Di Zhao
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  8. Xijing Chen
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Corresponding author

Correspondence to Xijing Chen.

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Zhang, Q., Yang, W., Song, H. et al. Tissue distribution and ontogeny of multidrug resistance protein 2, a phosphatidylcholine translocator, in rats. Eur J Drug Metab Pharmacokinet 41, 87–91 (2016). https://doi.org/10.1007/s13318-014-0226-5

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  • DOI: https://doi.org/10.1007/s13318-014-0226-5

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