Studies on pharmacokinetics and bioavailability of aminophylline in partridge chickens

Abstract

Veterinary medicine plays a significant role in the development of animal husbandry. Drugs residual in food would follow the food-chain coming into human body, which might bring hidden dangers to people. Chicken is the prime source of meat food, whose quality is important for our life and health. Therefore, it is necessary to realize the withdrawal period and establish an efficient, sensitive and accurate method for monitoring the metabolic process of drugs in chicken body. In this paper, the pharmacokinetics of aminophylline in partridge chicken after intravenous and oral administration was investigated using a sensitive high-performance liquid chromatography method. Plasma concentration–time profiles of aminophylline were analyzed by a non-compartmental model using Topfit 2.0. Following intravenous and oral administration, the peak concentrations (C _max) were found to be (16.5 ± 3.0) µg/mL at (0.08 ± 0) h and (7.4 ± 1.5) µg/mL at (1.83 ± 1.11) h, respectively. The elimination half-time (t _1/2) after intravenous and oral administration were, respectively, (13.1 ± 4.17) h and (11.65 ± 1.14) h. Areas under the plasma concentration–time curve (AUC) were (209.6 ± 22.8) µg h mL⁻¹(AUC_0–t ) and (219.5 ± 28.3) µg h mL⁻¹ (AUC_0→∞ ) after intravenous, and (165.1 ± 37.0) µg h mL⁻¹(AUC_0–t ) and (179.3 ± 35.6) µg h mL⁻¹ (AUC_0→∞ ) after oral administration. Mean retention time (MRT) after intravenous and oral administration were, respectively, (14.06 ± 0.86) and (15.27 ± 0.62) h. The total clearance rates (CL_tol) were (0.77 ± 0.10) mL min⁻¹ kg⁻¹ of intravenous and (0.97 ± 0.20) mL min⁻¹ kg⁻¹ of oral administration. The apparent distribution volume (V _d) was (0.87 ± 0.27) and (0.97 ± 0.20) L kg⁻¹, respectively, for intravenous and oral administration. The absolute bioavailability (F) after oral administration was (83.1 ± 11.7) %. The results showed that aminophylline in partridge chickens had a longer elimination half-time, a smaller clearance rate, as well as a higher absolute bioavailability for oral administration. Therefore, aminophylline in partridge chickens produced a long healing efficacy and oral administration can achieve a good absorption which could meet the requirement.