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Effect of Golgi phosphoprotein 2 (GOLPH2/GP73) on autophagy in human hepatocellular carcinoma HepG2 cells

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Tumor Biology

Abstract

This study aims to investigate the effect of Golgi Protein 73 (GP73) on autophagy in human hepatoma line cells HepG2. We investigated the functional effects of GP73 on autophagy in hepatoma cell line HepG2 using immunofluoscence staining, Western blotting and real-time PCR. Our data showed that specific small interference RNA (siRNA) notably induced formation of autophagic vacuoles. In addition, upregulation of GP73 significantly inhibited formation of starvation-induced LC3-positive structures. We provide the first experimental evidence to show that GP73 may play an important role in the inhibitory regulation of autophagy. Therefore, our data suggest a new molecular mechanism for GP73-related hepatoma progression.

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Abbreviations

GP73:

73-kd Golgi protein (Genbank # AF236056)

LC3:

Microtubule-associated protein 1 light chain 3 (MAP1LC3)

SDS:

Sodium dodecyl sulfate

PBS:

Phosphate-buffered saline

DMEM:

Dulbecco’s modified Eagle’s medium

EBSS:

Earle’s balanced salt solution

FBS:

Fetal bovine serum

DAPI:

4,6-Diamidino-2-phenylindole

ANOVA:

analysis of variance

ATG:

autophagy-related gene

HCC:

Hepatocellular carcinoma

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Acknowledgments

The authors gratefully acknowledge the financial support by the Project 2013C14011 from Department of Science and Technology of Zhejiang Province, and the Project of National Essential Drug Research and Development of China (2013ZX09506015).

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None

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Author notes

  1. Lin-Fu Zhou

    Present address: Faculty of Basic Medicine, Medicine School of Zhejiang University, 866 Yuhangtang Road, Post-box 40, Hangzhou, 310058, Zhejiang Province, China

Authors and Affiliations

  1. Medical Biotechnology Laboratory, Zhejiang University, Hangzhou, 310058, China

    Yuan-Yuan Zhou, Jun-Chang Jiang & Lin-Fu Zhou

  2. Zhejiang Cancer Hospital, No. 38. Guangji Road, Banshan Bridge, Gongsu District, Hangzhou, 310022, China

    Jun You

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  1. Yuan-Yuan Zhou
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Correspondence to Lin-Fu Zhou.

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Zhou, YY., Jiang, JC., You, J. et al. Effect of Golgi phosphoprotein 2 (GOLPH2/GP73) on autophagy in human hepatocellular carcinoma HepG2 cells. Tumor Biol. 36, 3399–3406 (2015). https://doi.org/10.1007/s13277-014-2974-x

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  • DOI: https://doi.org/10.1007/s13277-014-2974-x

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