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Ameliorative effect of chlorin e6-mediated photodynamic therapy on DNCB-induced atopic dermatitis-like skin lesions in mice

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Abstract

Backgrounds: Atopic dermatitis (AD) is a chronic, inflammatory, and relapsing skin disorder that occurs due to a Th1/Th2 cytokine imbalance and an elevation of immunoglobulin E (IgE) levels. We investigated whether chlorin e6 (Ce6)-mediated photodynamic therapy (PDT), which is commonly used for the clinical treatment of several cancers and non-neoplastic skin disorders, has a therapeutic effect on AD.

Methods: β-hexosaminidase assay was performed in RBL-2H3 stimulated by DNP-BSA. Total IgE levels and serum cytokine levels were analyzed by ELISA assay. Eosinophils and mast cells were analyzed by hematoxylin- eosin stain and toluidine blue stain on ear of AD mouse model.

Results: Ce6-mediated PDT significantly reduced the secretion of β-hexosaminidase in DNP-BSA-stimulated RBL-2H3 cells. In a DNCB-induced AD-like mouse model, serum IFN-γ was found to be elevated in conjunction with reduced IL-4 following Ce6-mediated PDT. The DNCB-induced serum IgE level was also reduced following Ce6-mediated PDT. In addition, DNCB-induced infiltration of eosinophils and mast cells was decreased by Ce6-mediated PDT in the mouse ear.

Conclusion: Ce6-mediated PDT might be used as a potent therapeutics for AD via restoring Th1/Th2 balance and reducing over expressed immune cells.

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Acknowledgements

This work was supported by Soonchunhyang University.

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Authors and Affiliations

  1. Department of Medical Science, Soonchunhyang University, 22 Soonchunhyang-ro, Asan, Chungnam, 31538, Republic of Korea

    A-Reum Ryu & Mi-Young Lee

  2. Department of Medical Biotechnology, Soonchunhyang University, 22 Soonchunhyang-ro, Asan, Chungnam, 31538, Republic of Korea

    Mi-Young Lee

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  1. A-Reum Ryu
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  2. Mi-Young Lee
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Correspondence to Mi-Young Lee.

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Conflict of Interest

A-Reum Ryu & Mi-Young Lee declare that they have no conflicts of interest.

Human and animal rights

The experimental procedure followed the actual law of animal protection that was approved by the Institutional Animal Care and Use Committee of Soonchunhyang University, Korea.

Author’s contributions

A-Reum carried out the experiment and Mi-Young Lee supervised the project. All authors discussed the results and contributed to the final manuscript.

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Ryu, AR., Lee, MY. Ameliorative effect of chlorin e6-mediated photodynamic therapy on DNCB-induced atopic dermatitis-like skin lesions in mice. Mol. Cell. Toxicol. 15, 265–270 (2019). https://doi.org/10.1007/s13273-019-0030-z

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  • DOI: https://doi.org/10.1007/s13273-019-0030-z

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