Abstract
Background
A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.
Objectives
We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.
Methods
We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).
Results
Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.
Conclusion
Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.
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Acknowledgements
This work was supported by a grant from the Ministry of Education, Republic of Korea (NRF-2015R1D1A1A01056650).
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The animal protocol and sample extraction method in this study were reviewed by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) for the ethical procedures and scientific care (approval number PNU-2013-0411).
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Kim, SW., Jo, A., Im, J. et al. Expression analysis of miR-221-3p and its target genes in horses. Genes Genom 41, 459–465 (2019). https://doi.org/10.1007/s13258-018-00778-3
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DOI: https://doi.org/10.1007/s13258-018-00778-3