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The effect of iron dextran on the transcriptome of pig hippocampus

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Abstract

The chain of events resulting from oxidative stress is still barely understood. The hippocampus is particularly vulnerable to oxidative stress. With the emergence of high-throughput sequencing, many different biological components can be discovered simultaneously. Using RNA-seq technology, we conducted a whole-transcriptome analysis of the hippocampi of piglets exposed to iron dextran (FeDex), a potent inducer of oxidation stress. The total hippocampal RNA from the piglets was sequenced, and 11.5 Gb of sequencing data were obtained. Regulatory molecules involved in oxidative stress were determined through the identification of 362 differentially expressed genes (DEGs). The functional analysis revealed that these DEGs were primarily involved in the defense response and responses to external stimuli. Furthermore, 76 neuropeptide gene transcripts and 60 neuropeptide receptor gene transcripts were identified in the hippocampus. Of these transcripts, the mRNA levels of six neuropeptide genes were significantly changed. Overall, this study describes the first use of RNA-seq to elucidate the transcriptomic changes in the piglet hippocampus caused directly by the injection of iron dextran, which enabled the characterization of the transcriptional response triggered by oxidative stress.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (No. 31201807), Fundamental Research Funds for the Central Universities (No. XDJK2016C055), Doctoral Fund of Southwest University (No. 2013Bsro5), Youth fund of Rongchang Campus of Southwest University (No. 20700416), Natural Science Foundation of Chongqing (No. cstc2016jcyjA0282), Science and Technology Innovation Foundation of Social Livelihood of the People of Chongqing (No. cstc2016shmszx80063) and Innovation Teams Foundation in University (No. CXTDG201602003). LG conceived of the study and developed the study design. LG carried out the analysis and drafted the manuscript. BY and HM participated in the study design and drafting of the manuscript. All authors read and approved the final manuscript.

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  1. The Department of Veterinary Medicine, Rongchang Campus, Southwest University, Rongchang, Chongqing, 402460, China

    Ling Gan, Binyu Yang & Hongyuan Mei

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  1. Ling Gan
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  2. Binyu Yang
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  3. Hongyuan Mei
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Correspondence to Ling Gan.

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Ling Gan declares that she has no conflict of interest. Binyu Yang declares that he has no conflict of interest. Hongyuan Mei declares that he has no conflict of interest.

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The experimental procedures followed the actual law of animal protection that was approved by the Animal Care Advisory Committee of Southwest University, China.

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Gan, L., Yang, B. & Mei, H. The effect of iron dextran on the transcriptome of pig hippocampus. Genes Genom 39, 1–14 (2017). https://doi.org/10.1007/s13258-016-0469-4

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