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Region-specific changes in the distribution of transient receptor potential vanilloid 4 channel (TRPV4) in the central nervous system of Alzheimer’s disease model mice

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Abstract

Transient receptor potential vanilloid type 4 (TRPV4) channel is expressed in the central nervous system and its role in development of Alzheimer’s disease (AD) is largely unknown. To identify AD-related changes in the TRPV4 channel distribution in the central nervous system, we investigated the distribution and level changes of TRPV4 in brains of AD model mice. The expressions of TRPV4 in the brain of control mice, early stage and late stage AD model mice were compared using immunohistochemistry with antibodies recognizing TRPV4 on free floating sections and in addition we performed western blotting to supplement our findings. TRPV4 immunoreactivity was significantly increased in the cerebral cortex, hippocampal formation, striatum and thalamus of AD model mice compared with control mice. In the cerebral cortex, TRPV4 immunoreactivity was significantly increased in pyramidal cells of early stage and late stage AD model mice. In addition, TRPV4 immunoreactivity was increased in the hippocampal formation, striatum and thalamus of late stage AD model mice. This is the first demonstration of AD-related increases in TRPV4 expression in the brain and it may provide useful data for investigating the pathogenesis of AD-related neurodegenerative diseases. The regulation of TRPV4 in AD mouse model and its functional significance require further investigation.

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Acknowledgments

The Korea Foundation for the Advancement of Science and Creativity (KOFAC) Grant funded by the Korea government (MEST).

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Authors and Affiliations

  1. Department of Biology, School of Life Sciences, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, 361-763, Republic of Korea

    Jae Chul Lee & Soo Young Choe

  2. Department of Anatomy, Seoul National University College of Medicine, Seoul, Republic of Korea

    Jae Chul Lee

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  1. Jae Chul Lee
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  2. Soo Young Choe
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Correspondence to Soo Young Choe.

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The authors have no conflicts of interest to disclose.

Ethical approval

All animal experiments were approved by the appropriate Institutional Review Boards of the Seoul National University College of Medicine (Seoul, Korea; SNU-140212-2) and conducted in accordance with National Institutes of Health Guide for the Care Use of Laboratory Animals (NIH publication No. 86-23, revised in 1996).

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Lee, J.C., Choe, S.Y. Region-specific changes in the distribution of transient receptor potential vanilloid 4 channel (TRPV4) in the central nervous system of Alzheimer’s disease model mice. Genes Genom 38, 629–637 (2016). https://doi.org/10.1007/s13258-016-0389-3

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  • DOI: https://doi.org/10.1007/s13258-016-0389-3

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