Avoid common mistakes on your manuscript.
Dear Editor,
The standard data analysis of genome-wide association study (GWAS) is based on single SNP (single nucleotide polymorphism), thus it ignores combined effect of modest SNPs/genes. To solve this problem, pathway-based analysis (PBA) has been introduced to GWAS data analysis. PBA aims to identify biological functions and mechanisms associated with complex trait (Wang et al., 2007; Wang et al., 2010). By now it has been one of the key ways to interpret GWAS data (Wang et al., 2010).
The results of PBA are trait-associated pathways, which represent combined effect of modest genes. Further validation study needs to explore candidate causative SNPs from the PBA-identified pathways, by annotating the SNPs of the genes involved in pathways based on genomic features including protein coding features and non-coding features. For coding features, the SNPs impacting protein functions (such as deleterious non-synonymous sites) have been widely investigated, and the information has been well collected in some databases like Ensembl (Flicek et al., 2014). Meanwhile, to assign the biochemical function of the non-coding part of human genome (particular functional elements for gene expression regulation), the Encyclopedia of DNA Elements (ENCODE) project (Bernstein et al., 2012) has identified plenty of regulatory regions, like DNase I hypersensitive sites (DHSs) and transcription factor binding sites (TFBSs) in human genome. Particularly, the result of ENCODE indicates that most of the SNPs identified by GWASs are enriched within non-coding functional elements, with a majority residing in or near ENCODE-defined regions, including DHSs and TFBSs across several cell types (Bernstein et al., 2012). By now there have been several tools, such as GenomeRunner (Dozmorov et al., 2012) and GREAT (McLean et al., 2010), which can annotate and analyze the non-coding genomic features for input genomic regions. However, these tools are general tools and not specific for trait-associated pathways identified from GWAS. Furthermore, coding region annotation and linkage disequilibrium (LD), which is the basic concept of GWAS, need to be considered. On the other hand, except for genomic features, expression quantitative trait loci (eQTLs) analysis is widely utilized to interpret biological mechanisms of GWAS-identified variants (Cookson et al., 2009). Taken together, a PBA tool combined with solutions for functional analysis (considering all above issues) of SNPs in PBA-identified pathways associated with trait will provide an objective and comprehensive way to interpret GWAS data.
In our previous work, we have developed a PBA web server, i-GSEA4GWAS (improved gene set enrichment analysis for GWAS), which detects pathways associated with traits by applying an improved gene set enrichment analysis (i-GSEA) (Zhang et al., 2010). Here, we report a new version, i-GSEA4GWAS v2, which is featured by implementing both i-GSEA and follow-up functional analysis for SNPs in trait-associated pathways identified by i-GSEA as well as their linkage disequilibrium (LD) proxies. The functional analysis of i-GSEA4GWAS v2 is based on putative functional SNP annotation data from Ensembl, regulatory regions from ENCODE and eQTLs data. Both annotation analysis and enrichment analysis were conducted for each type of functional elements. Data sources used for functional analysis were shown in Tables S1–3. Details about the annotation and enrichment analysis methods are in Materials and Methods section in Supplementary Materials. Fig. 1 shows the analytical framework of i-GSEA4GWAS v2.
I-GSEA4GWAS v2 is freely available at http://gsea4gwas-v2.psych.ac.cn/. It supports all major browsers. The main options of it are in Table 1. There is no any restriction to use it by academics and non-academics. It is written in Java and JSP and distributed using Apache and Tomcat web servers. I-GSEA4GWAS v2 was run in an upgraded web server (DELL R910 with 256G memory, 40 core CPUs and almost 10T storage) than i-GSEA4GWAS to improve the computing speed and work load. It is platform independent and easy to use by genetic and biological researchers; web browser is the only requirement to use it.
We applied i-GSEA4GWAS v2 to analyze 312,565 SNP P-values of a schizophrenia GWAS with 871 schizophrenia cases and 863 healthy controls (all of European origin) at discovery stage (Need et al., 2009). The program maps the SNPs to the nearest genes within 20 kb upstream/downstream, searches KEGG (Kanehisa et al., 2014), BioCarta (http://www.biocarta.com/), and GO (Ashburner et al., 2000) for trait-associated pathways, and extracts linkage disequilibrium (LD) proxies for functional analysis from 1000 Genomes EUR. The result identified 19 pathways with FDR < 0.05 (Table S4). Among these 19 pathways, one (‘antigen processing and presentation’) was significantly enriched in Ensembl other putative functional sites, 8 of them were significantly enriched in at least one track of ENCODE regulatory elements and 14 of them were significantly enriched in eQTL, which indicated most SNPs in these pathways were in non-coding regions which may regulate the gene expression (Bernstein et al., 2012). For ‘antigen processing and presentation’, it is suggested that in schizophrenia, cellular mechanisms that are involved in antigen processing and presentation could be less efficient (Craddock et al., 2007). Functional analysis for the significant SNPs and their LD proxies in this pathway indicates that LD proxies (rs9260107 and rs9260118) of rs2860580 in HLA-A, LD proxies (rs2072895 and rs2844846) of rs1362126 in HLA-F and LD proxy (rs3100139) of rs2254835 in B2M are annotated as splice region; LD proxy (rs2072895) of rs1362126 in HLA-F is annotated as deleterious. The three genes are all related with major histocompatibility complex (MHC) class I molecules, which is consistent with the recent findings about the contribution of MHC region site to schizophrenia (Ripke et al., 2013). Four schizophrenia-associated pathways ‘potassium ion transport’, ‘regulation of heart contraction’, ‘voltage gated potassium channel complex’, and ‘potassium channel activity’ were enriched in ENCODE FARE track “wgEncodeOpenChromFaireMedulloPk” (a cell line of brain, as shown in Table S5), indicating many SNPs in these pathways may regulate the gene expression in brain. These functional SNPs and genes we identified may lead important function in schizophrenia and deserve further validation.
In summary, this release of i-GSEA4GWAS v2 adds the important features towards an objective and comprehensive GWAS data interpretation, namely functional analysis for SNPs in trait-associated pathways. The functional analysis implemented in our web server for SNPs in trait-associated pathways considers linkage disequilibrium (LD) information, three categories of features (coding, non-coding and eQTLs) and enrichment analysis, which is a very comprehensive tool/module in comparison to available web-based tools for functional analysis (Table S6). The functional analysis result would facilitate to understand all kinds of main features (coding, non-coding and eQTLs) of the pathway related SNPs and select candidate causal SNPs, which will further contribute to the interpretation of GWAS data. To our knowledge, this is the first effort that SNP functional analysis is implemented in a PBA tool for GWAS. In future research, we will continue to update i-GSEA4GWAS v2 with latest pathways and annotation data of genomic features.
References
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT et al (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25:25–29
Bernstein BE, Birney E, Dunham I, Green ED, Gunter C, Snyder M (2012) An integrated encyclopedia of DNA elements in the human genome. Nature 489:57–74
Cookson W, Liang L, Abecasis G, Moffatt M, Lathrop M (2009) Mapping complex disease traits with global gene expression. Nat Rev Genet 10:184–194
Craddock RM, Lockstone HE, Rider DA, Wayland MT, Harris LJ, McKenna PJ, Bahn S (2007) Altered T-cell function in schizophrenia: a cellular model to investigate molecular disease mechanisms. PLoS One 2:e692
Dozmorov MG, Cara LR, Giles CB, Wren JD (2012) GenomeRunner: automating genome exploration. Bioinformatics 28:419–420
Flicek P, Amode MR, Barrell D, Beal K, Billis K, Brent S, Carvalho-Silva D, Clapham P, Coates G, Fitzgerald S et al (2014) Ensembl 2014. Nucleic Acids Res 42:D749–D755
Kanehisa M, Goto S, Sato Y, Kawashima M, Furumichi M, Tanabe M (2014) Data, information, knowledge and principle: back to metabolism in KEGG. Nucleic Acids Res 42:D199–D205
McLean CY, Bristor D, Hiller M, Clarke SL, Schaar BT, Lowe CB, Wenger AM, Bejerano G (2010) GREAT improves functional interpretation of cis-regulatory regions. Nat Biotechnol 28:495–501
Need AC, Ge D, Weale ME, Maia J, Feng S, Heinzen EL, Shianna KV, Yoon W, Kasperaviciute D, Gennarelli M et al (2009) A genome-wide investigation of SNPs and CNVs in schizophrenia. PLos Genet 5:e1000373
Ripke S, O’Dushlaine C, Chambert K, Moran JL, Kahler AK, Akterin S, Bergen SE, Collins AL, Crowley JJ, Fromer M et al (2013) Genome-wide association analysis identifies 13 new risk loci for schizophrenia. Nat Genet 45:1150–1159
Wang K, Li M, Bucan M (2007) Pathway-based approaches for analysis of genomewide association studies. Am J Hum Genet 81:1278–1283
Wang K, Li M, Hakonarson H (2010) Analysing biological pathways in genome-wide association studies. Nat Rev Genet 11:843–854
Zhang K, Cui S, Chang S, Zhang L, Wang J (2010) i-GSEA4GWAS: a web server for identification of pathways/gene sets associated with traits by applying an improved gene set enrichment analysis to genome-wide association study. Nucleic Acids Res 38:W90–W95
FOOTNOTES
This work was supported by National Natural Science Foundation of China (Grant No. 81101545) and Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-J-8).
Kunlin Zhang, Suhua Chang, Liyuan Guo, and Jing Wang declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by the any of the authors.
Author information
Authors and Affiliations
Corresponding author
Additional information
Kunlin Zhang and Suhua Chang have contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
About this article
Cite this article
Zhang, K., Chang, S., Guo, L. et al. I-GSEA4GWAS v2: a web server for functional analysis of SNPs in trait-associated pathways identified from genome-wide association study. Protein Cell 6, 221–224 (2015). https://doi.org/10.1007/s13238-014-0114-4
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13238-014-0114-4