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Fabrication of recombinant human bone morphogenetic protein-2 coated porous biphasic calcium phosphate-sodium carboxymethylcellulose-gelatin scaffold and its In vitro evaluation

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Abstract

Fabrication of bone substitutes, which are a combination of bio-ceramics and bio-polymer, is performed to meet the demand for bone regeneration after fracture or disease. In this study, sodium carboxymethylcellulosegelatin (NaCMC-GEL) hydrogel scaffold where the ratio of NaCMC and GEL was 1:2 and biphasic calcium phosphate (BCP) loaded NaCMC-GEL where the ratio of BCP, NaCMC and GEL was 1:1:2 hydrogel scaffold were fabricated successfully by the freeze-drying method. These hydrogel scaffolds were crosslinked by 0.75wt% genipin solution to check the swelling and ensure optimum degradation. Then BCP-NaCMC-GEL hydrogel was coated with recombinant human bone morphogenetic protein-2 (rhBMP-2). Detailed morphological and material characterization, such as porosity, micro-structural analysis, and chemical constituents of NaCMC-GEL and BCP-NaCMC-GEL hydrogel scaffolds was carried out. The study demonstrates that these hydrogel scaffolds have a porous structure and the pore size is optimum for bone tissue regeneration. PBS uptake and degradation behavior of the NaCMC-GEL, BCP-NaCMC-GEL and BMP-2-BCP-NaCMC-GEL hydrogel scaffolds were also observed. BMP-2-BCP-NaCMCGEL hydrogel scaffold showed higher cell viability, cell attachment, and proliferation of pre-osteoblast MC3T3-E1 cells than the NaCMC-GEL, BCP-NaCMC-GEL scaffolds, which was confirmed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, live-dead assay, and immunofluorescence assay. The BMP-2 release profile from BMP-2-BCP-NaCMC-GEL scaffold was also shown. The observations show that BMP-2-coated BCPNaCMC-GEL hydrogel scaffold is a promising material for bone tissue regeneration.

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Authors and Affiliations

  1. Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Chungnam, 330-090, Korea

    Avik Sarker, Nguyen Thuy Ba Linh & Byong Taek Lee

  2. Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Chungnam, 330-090, Korea

    Nguyen Thuy Ba Linh & Byong Taek Lee

  3. Department of Surgery, Soonchunhyang University Cheonan Hospital, 31Soonchunhyang 6gil, Dongnam-gu, Chungnam, 330-721, Korea

    Hae Il Jung

  4. Department of Exercise Prescription, Konyang University, 121,Dahak-ro, Non San City, Chungnam, 320-711, Korea

    Hyun Seok Seo

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  1. Avik Sarker
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  2. Nguyen Thuy Ba Linh
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  3. Hae Il Jung
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  4. Hyun Seok Seo
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  5. Byong Taek Lee
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Correspondence to Byong Taek Lee.

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Sarker, A., Linh, N.T.B., Jung, H.I. et al. Fabrication of recombinant human bone morphogenetic protein-2 coated porous biphasic calcium phosphate-sodium carboxymethylcellulose-gelatin scaffold and its In vitro evaluation. Macromol. Res. 22, 1297–1305 (2014). https://doi.org/10.1007/s13233-014-2185-8

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  • DOI: https://doi.org/10.1007/s13233-014-2185-8

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