Skip to main content
Log in

Immobilization of glucose oxidase attached to new nanospheres including azomethine

  • Articles
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

New nanosphere supports were prepared and characterized for the immobilization of glucose oxidase from Aspergillus niger. Nanoparticles ((APS-tio), (APS-tioCH3), and (APS-tioCl)) modified (aminomethyl)polystyrene (APS) with some tyophenealdehyde derivatives were synthesized by means of condensation and we investigated the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on them. Modified polystyrene was characterized by IR spectra, gel permeation chromatography and scanning electron microscopy. Immobilized GOx on (APS-tioCH3) showed a optimum pH, whereas immobilized GOx on (APS-tio) and (APS-tioCl) performed the optimum condition at two pHs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. E. Aynaci, N. Sari, and H. Tümtürk, Artif. Cells Blood Substit. Immobil. Biotechnol., 39, 259 (2011).

    Article  Google Scholar 

  2. V. Erduran, H. Tümtürk, E. Aynaci, and N. Sari, J. Macromol. Sci. A, 50, 871 (2013).

    Article  CAS  Google Scholar 

  3. A. E. David, N. S. Wang, V. C. Yang, and A. J. Yang, J. Biotechnol., 125, 395 (2006).

    Article  CAS  Google Scholar 

  4. E. Bozkir, N. Sari, and H. Ögütcü, J. Inorg. Organomet. Polym., 22, 1146 (2012).

    Article  CAS  Google Scholar 

  5. F. Hanf and P. Koehler, J. Sci. Food Agric., 86, 1699 (2006).

    Article  Google Scholar 

  6. J. Yu, S. Liu, and H. Ju, Biosens. Bioelctron., 19, 401 (2003).

    Article  CAS  Google Scholar 

  7. H. Arslan, M. Özdemir, and H. Zengin, Int. J. Electrochem. Sci., 7, 10205 (2012).

    CAS  Google Scholar 

  8. J. Ge, D. Lu, Z. Liu, and Z. Lui, Biochem. Eng. J., 44, 53 (2009).

    Article  CAS  Google Scholar 

  9. M. Moyo, J. O. Okonkwo, and M. N. Agyei, Sensors (Basel), 12, 923 (2012).

    Article  Google Scholar 

  10. S. Aksoy, H. Tumturk, and N. Hasirci, J. Biotechnol., 60, 37 (1998).

    Article  CAS  Google Scholar 

  11. J. M. Guisan, Immobilization of Enzymes and Cells, Methods in Biotechnology, Humana Press Inc., Totowa, 2, 14 (2006).

    Google Scholar 

  12. J. Huang, R. Zhao, H. Wang, W. Zhao, and L. Ding, Biotechnol. Lett., 32, 817 (2010).

    Article  CAS  Google Scholar 

  13. C. X. Guo and C. M. Li, Phys. Chem. Chem. Phys., 12, 12153 (2010).

    Article  CAS  Google Scholar 

  14. F. Arslan, S. Ustabasi, and H. Arslan, Sensors, 11, 8152 (2011).

    Article  CAS  Google Scholar 

  15. M. C¸il, A. E. Büyükbayram, S. Kiralp, L. Toppare, and Y. Yagci, Int. J. Bio. Macromol., 41 49 (2007).

    Article  Google Scholar 

  16. X. H. Xu, G. Leiren, J. Cheng Q. Liu, D. Guangli, and Q. Chen, J. Mater. Sci., 41 3147 (2006).

    Article  CAS  Google Scholar 

  17. P. Trinder, Ann. Clin. Biochem., 6, 24 (1969).

    Article  CAS  Google Scholar 

  18. N. Sari and S. Özcan, Chinese J. Polym. Sci., 27, 675 (2009).

    Article  CAS  Google Scholar 

  19. N. Sari and N. Yüzüak, J. Inorg. Organomet. Polym. Mater., 16, 259 (2006).

    Article  CAS  Google Scholar 

  20. R. M. Silverstein and F. X. Webster, Spectrometric Identification of Organic Compounds, 6th ed., John Wiley and Sons, Inc., New York, 1997, p 86.

    Google Scholar 

  21. N. Sari, E. Antepli, D. Nartop, and N. K. Yetim, Int. J. Mol. Sci., 13, 11870 (2012).

    Article  CAS  Google Scholar 

  22. X. Wang, K.-X Zhu, and H.-M. Zhou, Int. J. Mol. Sci., 12, 3042 (2011).

    Article  CAS  Google Scholar 

  23. Ö. Çolak, A. Yasar, S. Çete, and F. Arslan, Artif. Cells Blood Substit. Immobil. Biotechnol., 40, 354 (2012).

    Article  Google Scholar 

  24. M. Görgülü, S. Çete, H. Arslan, and A. Yasar, Artif. Cells Blood Substit. Immobil. Biotechnol., 41, 327 (2013).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nurşen Sarı.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Özdem, N., Özkan, E.H., Sarı, N. et al. Immobilization of glucose oxidase attached to new nanospheres including azomethine. Macromol. Res. 22, 1282–1287 (2014). https://doi.org/10.1007/s13233-014-2178-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-014-2178-7

Keywords

Navigation