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Enhanced gene delivery system using disulfide-linked chitosan immobilized with polyamidoamine

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Abstract

We developed a polyamidoamine (PAMAM) conjugated disulfide-linked chitosan (dsCS-PAM) for nonviral gene delivery. At weight ratio of 128, dsCS and dsCS-PAM showed positive zeta potential of around 9.5 mV and 14.5 mV, respectively, demonstrating their ability to form complexes with plasmid DNA (pDNA) for cell transfection. CS-PAM and dsCS-PAM formed complexes with pDNA in a reducing environment containing 5 mM DLdithiothreitol (DTT) at weight ratio 32 and 16, respectively. PAMAM conjugated chitosan derivatives, dsCS-PAM and CS-PAM, showed higher in vitro transfection efficiency than unconjugated derivatives dsCS and CS. Approximately 80% of A549 and 293 cells were viable in the presence of up to 100 μg/mL of dsCS-PAM. The results of this study demonstrate that dsCS-PAM has the potential to be a safe gene delivery carrier.

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References

  1. G. D. Schmidt-Wolf and I. G. H. Schmidt-Wolf, Trends Mol. Med., 9, 67 (2003).

    Article  CAS  Google Scholar 

  2. V. Vijayanathan, T. Thomas, and T. J. Thomas, Biochemistry, 41, 14085 (2002).

    Article  CAS  Google Scholar 

  3. S. Mao, W. Sun, and T. Kissel, Adv. Drug Deliv. Rev., 62, 12 (2010).

    Article  CAS  Google Scholar 

  4. I. Kim, S. Seo, H. Moon, M. Yoo, I. Park, B. Kim, and C. Cho, Biotechnol. Adv., 26, 1 (2008).

    Article  CAS  Google Scholar 

  5. M. Huang, C. Fong, E. Khor, and L. Lim, J. Control. Release, 106, 391 (2005).

    Article  CAS  Google Scholar 

  6. C. Choi, D. Kim, M. Jang, T. Kim, M. Jang, and J. Nah, J. Appl. Polym. Sci., 102, 3545 (2006).

    Article  CAS  Google Scholar 

  7. F. Kong, G. Liu, B. Sun, S. Zhou, A. Zuo, R. Zhao, and D. Liang, Int. J. Pharm., 422, 445 (2012).

    Article  CAS  Google Scholar 

  8. W. E. Rudzinski and T. M. Aminabhavi, Int. J. Pharm., 399, 1 (2010).

    Article  CAS  Google Scholar 

  9. P. Holzerny, B. Ajdini, W. Heusermann, K. Bruno, M. Schuleit, L. Meinel, and Keller, J. Control. Release, 157, 297 (2012).

    Article  CAS  Google Scholar 

  10. M. Lavertu, S. Méthot, N. Tran-Khanh, and M. D. Buschmann, Biomaterials, 27, 4815 (2006).

    Article  CAS  Google Scholar 

  11. C. Wiegand, D. Winter, and U. C. Hipler, Skin Pharmacol. Physiol., 23, 164 (2010).

    Article  CAS  Google Scholar 

  12. P. Opanasopit, J. Tragulpakseerojn, A. Apirakaramwong, T. Ngawhirunpat, and T. Rojanarata, Int. J. Pharm., 410, 161 (2006).

    Article  CAS  Google Scholar 

  13. R. M. Ramesan and C. P. Sharma, Nanomedicine, 7, 5 (2012).

    Article  CAS  Google Scholar 

  14. H. Jiang, Y. Kim, R. Arote, J. Nah, M. Cho, Y. Choi, T. Akaike, and C. Cho, J. Control. Release, 117, 273 (2007).

    Article  CAS  Google Scholar 

  15. K. Wong, G. Sun, X Zhang, H. Dai, Y. Liu, C. He, and K. W. Leong, Bioconjug. Chem., 17, 152 (2006).

    Article  CAS  Google Scholar 

  16. K. Sarkar and P. P. Kundu, Macromolecules, 51, 859 (2012).

    Article  CAS  Google Scholar 

  17. Q. Peng, Z. Zhong, and R. Zhuo, Bioconjug. Chem., 19, 499 (2008).

    Article  CAS  Google Scholar 

  18. Q. Zhao, J. Chen, M. Han, W. Liang, Y. Tabata, and J. Gao, J. Biosci. Bioeng., 105, 65 (2008).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, 84112, USA

    Kihoon Nam, Ji Won Park, Heidi Bark, Jing Han & Sung Wan Kim

  2. Department of Polymer Science and Engineering, Sunchon National University, Jeonnam, 540-742, Korea

    Jae-Woon Nah & Mi-Kyeong Jang

Authors
  1. Kihoon Nam
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  2. Ji Won Park
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  3. Heidi Bark
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  4. Jing Han
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  5. Jae-Woon Nah
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  6. Mi-Kyeong Jang
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  7. Sung Wan Kim
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Correspondence to Sung Wan Kim.

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These authors contributed equally to this work.

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Nam, K., Park, J.W., Bark, H. et al. Enhanced gene delivery system using disulfide-linked chitosan immobilized with polyamidoamine. Macromol. Res. 22, 370–376 (2014). https://doi.org/10.1007/s13233-014-2052-7

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  • DOI: https://doi.org/10.1007/s13233-014-2052-7

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