Abstract
We established in vitro augmentation of callus, organogenesis and photosynthetic pigments content in Artemisia annua using Piriformospora indica filtrate and by optimizing plant growth regulators on culture medium. In vitro raised plantlets from nodal explants showed the best shooting at MS + BAP (3 mg/l) while ½ MS + IBA (3 mg/l) was the best combination for rooting. Co-cultivation of nodal explants using P. indica was done just after root initiation. Leaf explants supplemented with MS + 2, 4 D (3 mg/l) showed the best callogenesis. Interaction of in vitro raised plantlets and callus with the fungus and the filtrate resulted in overall increase in plant biomass and chlorophyll contents. Within 15 days of inoculation, the fungus colonized with the root cortical region of the plantlet and showed an increase in growth parameters such as root (21 %) and shoot (37.2 %) length, fresh (35 %) and dry weight (3 %), and most importantly pigment contents i.e. chlorophyll a (2 %), chlorophyll b (0.3 %) and carotenoid (66 %). P. indica filtrate (1 μl/ml)-treated light green friable callus obtained from leaf explants using MS + 2,4 D (3 mg/l), showed the highest biomass within 35 days. Thus, the study demonstrated the symbiotic effect of P. indica as a biopriming agent for the overall growth of biomass in Artemisia annua.
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Abbreviations
- BAP:
-
6-Benzylaminopurine
- Kn:
-
Kinetin
- IBA:
-
Indole butyric acid
- MS:
-
Murashige and Shoog
- μl/l:
-
micro litre/litre
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Acknowledgments
The work was supported by Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India under the DBT’s Twinning Programme for the North East (No. BT. 45/NE/TBP/2010). The authors thank the funding agency for the financial support. We appreciate Prof. Ait Varma of Amity University, Noida, Uttar Pradesh, India for supplying the culture of mycorrhizal fungi and his helpful instructions.
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Key message: Co-cultivation of the P. indica culture filtrate with Artemisia annua can increase the growth and development of the plant which may have biotechnological application
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Baishya, D., Deka, P. & Kalita, M.C. In vitro co-cultivation of Piriformospora indica filtrate for improve biomass productivity in Artemisia annua(L.). Symbiosis 66, 37–46 (2015). https://doi.org/10.1007/s13199-015-0331-5
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DOI: https://doi.org/10.1007/s13199-015-0331-5