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Mycorrhizal colonization decreases respiration of common bean nodulated root in hydroaeroponic culture

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Abstract

The bean-rhizobia symbiosis allows atmospheric nitrogen fixation through nodule formation. Nevertheless, nodule establishment in Mediterranean areas is subjected to various biotic and abiotic constraints such as phosphorus soils deficiency. This study compares plant-growth response to moderate (75 μmol KH2PO4 plant−1 week−1) versus severe phosphorus deficiency (30 μmol KH2PO4 plant−1 week−1) after inoculation with Rhizobium tropici CIAT 899 and Glomus intraradices of four Phaseolus vulgaris lines contrasting in P use efficiency (PUE) for their symbiotic nitrogen fixation (SNF) in hydroaeroponic culture. After 5 weeks of growth under glasshouse conditions, the oxygen consumption related to nitrogen fixation was measured on intact nodulated roots. The obtained results revealed that mycorrhizal colonization decreased the nodulated-roots O2 consumption of P. vulgaris under both P deficiencies although it increased the growth of all plant organs and the nodulation with a large genotypic variability. Moreover, mycorrhizal colonization was higher under severe P deficiency than under moderate one. In conclusion, the tripartite inoculation improved growth parameters under severe P-deficiency with a decrease in nodulated root O2 consumption.

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Abbreviations

AMF:

Arbascular mycorrhizal fungi

COP:

Critical oxygen pressure

Conr:

Root O2 consumption

DAS:

Days after sowing

EURS:

Efficiency in utilization of the rhizobial symbiosis

O2 :

Oxygen

Oex :

External O2

Oin :

Internal O2

P:

Phosphorus

pO2 :

O2 pressure

PUE:

P use efficiency

RIL:

Recombinant inbred lines

SNF:

Symbiotic nitrogen fixation

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Acknowledgments

This work was supported by AVERROES scholarship program for the stay of BENADIS Chahinez in Montpellier, the Grand Federative Project Fabatropimed of Agropolis Fundation, Montpellier, France, under the reference ID 1001-009, and PNR of Algeria. The authors thank Adnane Bargaz for his statistical help and Hélène Vailhe (INRA, Montpellier) for her technical assistance.

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Authors and Affiliations

  1. UMR 1222 Eco&Sols (Ecologie Fonctionnelle & Biogéochimie des Sols et des Agro-écosystèmes) CIRAD-INRA-IRD-SupAgro, Place Viala, Montpellier, France

    Chahinez Benadis, Siham Khoulassa & Jean Jacques Drevon

  2. Laboratoire de Biotechnologie des Rhizobia et Améliorations des Plantes, Faculté des Sciences, BP.16, Es-Senia, 31000, Oran, Algeria

    Chahinez Benadis, Abdelkader Bekki & Zineb Faiza Boukhatem

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  1. Chahinez Benadis
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  2. Abdelkader Bekki
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  3. Siham Khoulassa
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  4. Zineb Faiza Boukhatem
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  5. Jean Jacques Drevon
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Correspondence to Chahinez Benadis.

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Mycorrhizal colonization decreases the O2 consumption of nodulated roots of Phaseolus vulgaris under P-deficiency.

P-deficiency affects the growth parameters of P. vulgaris.

Genotypic variability of P. vulgaris induces differences in nodule O2 conductance.

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Benadis, C., Bekki, A., Khoulassa, S. et al. Mycorrhizal colonization decreases respiration of common bean nodulated root in hydroaeroponic culture. Symbiosis 62, 135–142 (2014). https://doi.org/10.1007/s13199-014-0277-z

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