Abstract
Comparative investigation of major phytoconstituents was performed from various parts of tea plant viz. apical bud, subtending 1st–5th leaf, stem, coarse leaves, flowers, fruits and roots. From the results of comparative RP-HPLC-DAD analysis it was found that underutilized tea parts especially coarse leaves, flowers and fruits contains abundant amount of phenolics (17.5%) and catechins (4–5%). From these underutilized tea plant parts the catechins were extracted and purified and then screened for their anticancer, immunomodulatory effect and antimicrobial activity against food borne pathogens. The results showed that tea fruit extract exhibited higher toxicity against oral cancer cells and also promotes proliferation of mice splenocytes. The results of antimicrobial studies revealed the inhibitory effect of these extracts against both gram positive and gram negative bacteria. These investigations clearly demonstrated that the underutilized tea plant parts could act as economical and sustainable bioresource of functionally active constituents which further lead to the development of new cost-effective nutraceuticals and other formulations.
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References
Anesini C, Graciela FE, Filip R (2008) Total polyphenol content and antioxidant capacity of commercially available tea (Camellia sinensis) in Argentina. J Agric Food Chem 56:9225–9229
Atoui AK, Mansouri A, Boskou G, Kefalas P (2005) Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem 89:27–36
Baptista J, Lima E, Paiva L, Andrade AL, Alves MG (2012) Comparison of Azorean tea theanine to teas from other origins by HPLC/DAD/FD. Effect of fermentation, drying temperature, drying time and shoot maturity. Food Chem 132:2181–2187
Cooper R, Morre DJ, Morre DM (2005) Medicinal benefits of green tea: part II. Review of anticancer properties. J Altern Complement Med 11:639–652
Dufresne CJ, Farnworth ER (2001) A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 12:404–421
Farhoosh R, Golmovahhed GA, Khodaparast MHH (2007) Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chem 100:231–236
Fernandez PL, Martín MJ, Gonzalez AG, Pablos F (2000) HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas. R Soc Chem Anal 125:421–442
Henriquez-Aedo K, Vega M, Aranda A (2013) Evaluation of tea functionality: determination of l-Theanine content in green and black teas by liquid chromatography. J Chil Chem Soc 58:1651–1654
Hsu SD, Singh BB, Lewis JB, Borke JL, Dickinson DP, Drake L, Caughman GB, Schuster GS (2002) Chemoprevention of oral cancer by green tea. Gen Dent 50:140–146
Lin YS, Tsai YJ, Tsay JS, Lin JK (2003) Factors affecting the levels of tea polyphenols and caffeine in tea leaves. J Agric Food Chem 51:1864–1873
Manzocco L, Anese M, Nicoli MC (1998) Antioxidant properties of tea extracts as affected by processing. LWT Food Sci Technol 31:694–698
Narukawa M, Morita K, Hayshi Y (2008) L-Theanine elicits an umami taste with inosine 5-monophosphate. J Sci Technol Dev 10:6–10
Nor Qhairul Izzreen MN, Mohd Fadzelly AB (2013) Phytochemicals and antioxidant properties of different parts of (Camellia sinensis) leaves from Sabah Tea Plantation in Sabah Malaysia. Int Food Res J 20:307–312
Obuchowicz J, Engelhardt UH, Donnelly K (2011) Flavanol database for green and black teas utilising ISO 14502-1 and ISO 14502-2 as analytical tools. J Food Comp Anal 24:411–417
Peng L, Song S, Shi S, Li J, Ye C (2008) An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species. J Food Comp Anal 21:559–563
Peterson J, Dwyer J, Bhagwat S, Haytowitz D, Holden J, Eldridge AL, Beecher G, Aladesanmi J (2005) Major flavonoids in dry tea. J Food Comp Anal 18:487–501
Quan PT, Hang TV, Ha NH, Gang BL (2007) Total polyphenols, total catechins content and DPPH free radical scavenger activity of several types of Vietnam commercial green tea. J Sci Technol Dev 10:6–10
Rana A, Bhangalia S, Singh HP (2013) A new phenylethanoid glucoside from (Jacaranda mimosifolia). Nat Prod Res 27:1167–1173
Rana A, Singh HP, Gulati A (2015) Concurrent analysis of theanine, caffeine, and catechins using hydrophobic selective C12 stationary phase. J Liq Chromatogr Relat Technol 38:709–715
Re R, Pellegrini N, Proteggente RC (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237
Rusaczonek A, Zebrowska M, Waszkiewicz-Robak B, Edyta S (2007) Evaluation of phenolic compounds content and antioxidant capacity of herbs. Polish J Food Nutr Sci 57(4):483–488
Sharma V, Gulati A, Ravindranath SD, Kumar V (2005) A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. J Food Comp Anal 18(6):583–594
Shazaan H, Lalithapriya J, Amy BH, Devang B, Dipti LB, Dana RC (2009) Select phytochemicals suppress human T-lymphocytes and mouse splenocytes suggesting their use in autoimmunity and transplantation. Nutr Res 29:568–578
Singh HP, Rana A (2014) An economical process for purification of free bio amino acids. CN104105685 A
Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 82:1107–1112
Stangl V, Dreger H, Stangl K, Lorenz M (2007) Molecular targets of tea polyphenols in the cardiovascular system. Int J Cardiovasc Res 73:348–358
Swain T, Hillis WE (1959) The phenolic constituents of (Prunus domestica) the quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68
Turkmen N, Velioglu YS, Sari F (2006) Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteau methods. Food Chem 99:835–841
Vuong QV, Golding JB, Nguyen MH, Roach PD (2010) Extraction and isolation of catechins from tea. J Sep Sci 33:3415–3428
Vuong QV, Bowyer MC, Roach PD (2011) L-Theanine: properties, synthesis and isolation from tea. J Sci Food Agric 91:1931–1939
Vuong QV, Golding JB, Nguyen MH, Roach PD (2012) Production of caffeinated and decaffeinated green tea catechin powders from underutilised old tea leaves. J Food Eng 110:1–8
Wang H, Helliwell K (2001) Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Res Int 34:223–227
Xu P, Bao Z, Gao J, Zhou T, Wang Y (2012) Optimization of extraction of phenolic antioxidants from tea (Camellia sinensis L.) fruit peel biomass using response surface methodology. Bioresources 7:2431–2443
Yang Z, Xu Y, Jie G, He P, Tu Y (2007) Study on the antioxidant activity of tea flowers (Camellia sinensis). Asia Pacific J Clin Nutr 16:148–152
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The authors acknowledge the financial support from Council of Scientific and Industrial Research (CSIR) New Delhi, India through BSC-0107 and MLP-0070. Kiran Rawat thanks D.S.T. for the award of S.R.F.
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Rana, A., Sharma, E., Rawat, K. et al. Screening and purification of catechins from underutilized tea plant parts and their bioactivity studies. J Food Sci Technol 53, 4023–4032 (2016). https://doi.org/10.1007/s13197-016-2406-6
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DOI: https://doi.org/10.1007/s13197-016-2406-6