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Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content

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Abstract

To elucidate the mechanism responsible for magnetic field induced seed invigoration in aged seeds an experiment was conducted on six year old garden pea seeds stored under controlled (20 °C and 40% RH) condition. Aged seeds were magnetoprimed by exposing to pulsed magnetic field (PMF) of 100 mT for 1 h in three pulsed modes. The 6 min on and off PMF showed significant improvement in germination (7.6%) and vigor (84.8%) over aged seeds. Superoxide and hydrogen peroxide production increased in germinating primed seeds by 27 and 52%, respectively, over aged seeds. Nicotinamide adenine dinucleotide (reduced) (NADH) peroxidase and superoxide dismutase involved in generation of hydrogen peroxide showed increased activity in PMF primed seeds. Increase in catalase, ascorbate peroxidase and glutathione reductase activity after 36 h of imbibition in primed seeds demonstrated its involvement in seed recovery during magnetopriming. An increase in total antioxidants also helped in maintaining the level of free radicals for promoting germination of magnetoprimed seeds. A 44% increase in level of protein carbonyls after 36 h indicated involvement of protein oxidation for counteracting and/or utilizing the production of ROS and faster mobilization of reserve proteins. Higher production of free radicals in primed seeds did not cause lipid peroxidation as malondialdehyde content was low. Lipoxygenase was involved in the germination associated events as the magnitude of activity was higher in primed aged seeds compared to aged seeds. Our study elucidated that PMF mediated improvement in seed quality of aged pea seeds was facilitated by fine tuning of free radicals by the antioxidant defense system and protein oxidation.

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Acknowledgements

The authors thank Indian Council of Agricultural Research, New Delhi, India for financial Grant under National Fund for Basic and Strategic Research in Agriculture, Grant Number: NFBSRA/PCN/AP-09/2006-07.

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Jyotsna Bhardwaj & Anjali Anand

  2. Regional Station, Indian Agricultural Research Institute, Karnal, India

    V. K. Pandita

  3. Indian Agricultural Research Institute, New Delhi, 110 012, India

    Shantha Nagarajan

Authors
  1. Jyotsna Bhardwaj
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  2. Anjali Anand
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  3. V. K. Pandita
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  4. Shantha Nagarajan
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Correspondence to Anjali Anand.

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Bhardwaj, J., Anand, A., Pandita, V.K. et al. Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content. J Food Sci Technol 53, 3969–3977 (2016). https://doi.org/10.1007/s13197-016-2392-8

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  • DOI: https://doi.org/10.1007/s13197-016-2392-8

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